ICCAS OpenIR
Speciation analysis of serum copper by ultrafiltration combined with graphite furnace atomic absorption spectrometry
Wang, ZH; Ma, HM; Ma, QL; Liang, SC
2001-02-01
Source PublicationCHINESE JOURNAL OF CHEMISTRY
ISSN1001-604X
Volume19Issue:2Pages:143-146
AbstractUltrafiltration combined with graphite furnace atomic absorption spectrometry (GFAAS) was used to study protein binding and speciation of copper in human serum. Ultrafiltration was carried out using a cell unit with ultrafiltration membranes having a nominal cut-off of 10,000 Dalton. The effects of various experimental factors including the kind and concentration of electrolyte, sample storage, pH, pressure and the preconditioning of the membranes on the speciation analysis of serum copper by ultrafiltration were examined. It was observed that 4.5 +/- 2.3% of the total copper in serum was ultrafiltrable and this value did not seem to be influenced by the total serum elemental concentration, the pH (6.5 - 10) and the pressure (I 1.5 kg/cm(2)). The preconditioning of the ultrafiltration system with 0.1 mol/L calcium nitrate can overcome the adsorption loss of copper effectively, and the addition of tris-HCl solution (pH 7.4) to serum accelerates the ultrafiltration. The present method was proved to be suitable for speciation analysis for its simplicity, rapidity, small sample requirement and easy control. The results obtained with the method are accurate and reliable.
KeywordUltrafiltration Speciation Analysis Serum Copper Graphite Furnace Atomic Absorption Spectrometry
Indexed BySCI
Language英语
WOS IDWOS:000166921000004
PublisherSCIENCE PRESS
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/77334
Collection中国科学院化学研究所
Corresponding AuthorWang, ZH
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wang, ZH,Ma, HM,Ma, QL,et al. Speciation analysis of serum copper by ultrafiltration combined with graphite furnace atomic absorption spectrometry[J]. CHINESE JOURNAL OF CHEMISTRY,2001,19(2):143-146.
APA Wang, ZH,Ma, HM,Ma, QL,&Liang, SC.(2001).Speciation analysis of serum copper by ultrafiltration combined with graphite furnace atomic absorption spectrometry.CHINESE JOURNAL OF CHEMISTRY,19(2),143-146.
MLA Wang, ZH,et al."Speciation analysis of serum copper by ultrafiltration combined with graphite furnace atomic absorption spectrometry".CHINESE JOURNAL OF CHEMISTRY 19.2(2001):143-146.
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