Spectroscopic studies and photodynamic actions of hypocrellin B in liposomes
Yu, CL; Chen, S; Zhang, MH; Shen, T
AbstractHypocrellin B (HB), a lipid-soluble natural pigment of perylenequinone derivative, is considered as potential photosensitizer for photodynamic therapy. Liposomes loaded with HE can constitute a simple model system, appropriate for better understanding the photodynamic action of HE in vivo. The steady-state absorption and emission spectra, quantum yield and lifetime of fluorescence of HE incorporated into egg L-a-phosphatidyl-choline (EPC) liposome were examined, The photochemical properties (Type I and/or Type II) of HE have also been studied in aqueous dispersions of small unilamellar liposomes of EPC using electron paramagnetic resonance and spectrophotometric methods, respectively. The quantum yield of O-1(2) generated by HE is ca 0.76 in chloroform solution and it did not change upon the incorporation of HE into liposomes of EPC, The superoxide anion radical was generated by the electron transfer from the anion radical of HE (HB.-) to oxygen. The disproportionation of O-2(.-) can generate H2O2 and ultimately the highly reactive (OH)-O-. via the Fenton reaction. It could be that the disproportionation proceeded too fast, so we could not detect O-2(.-) directly in aqueous dispersions of liposome EPC, Moreover, the self-sensitized photooxygenation of HE embedded in liposomes was studied, and almost fully (87%) inhibiting this reaction of HE by p-benzoquinone las the quencher of O-2(.-)) in aqueous dispersion of liposome EPC indicated that the radical mechanism (Type I) might be mainly involved in this oxygenation. All these findings suggested that the photodynamic action of HE proceeded via both Type-I and -II mechanisms, but Type-I mechanism might play a more important role in the aqueous dispersion.
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WOS IDWOS:000168578400005
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Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorZhang, MH
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Yu, CL,Chen, S,Zhang, MH,et al. Spectroscopic studies and photodynamic actions of hypocrellin B in liposomes[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY,2001,73(5):482-488.
APA Yu, CL,Chen, S,Zhang, MH,&Shen, T.(2001).Spectroscopic studies and photodynamic actions of hypocrellin B in liposomes.PHOTOCHEMISTRY AND PHOTOBIOLOGY,73(5),482-488.
MLA Yu, CL,et al."Spectroscopic studies and photodynamic actions of hypocrellin B in liposomes".PHOTOCHEMISTRY AND PHOTOBIOLOGY 73.5(2001):482-488.
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