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Ultrafast spectroscopy studies on the mechanism of electron transfer and energy conversion in the isolated pseudo ginseng, water hyacinth and spinach chloroplasts
Xu, SC; Sun, ZY; Ai, XC; Feng, J; Zhang, QY; Zhang, XK; Yu, F; Tang, CQ; Li, LB; Kuang, TY
2001-08-01
Source PublicationSCIENCE IN CHINA SERIES B-CHEMISTRY
ISSN1006-9291
Volume44Issue:4Pages:366-380
AbstractThe spectroscopy characteristics and the fluorescence lifetime for the chloroplasts isolated from the pseudo ginseng, water hyacinth and spinach plant leaves have been studied by absorption spectra, low temperature steady-state fluorescence spectroscopy and single photon counting measurement under the same conditions and by the same methods. The similarity of the absorption spectra for the chloroplasts at room temperature suggests that different plants can efficiently absorb light of the same wavelength. The fluorescence decays in PS II measured at the natural Q(A) state for the chloroplasts have been fitted by a three-exponential kinetic model. The three fluorescence lifetimes are 30, 274 and 805 ps for the pseudo ginseng chloroplast; 138, 521 and 1494 ps for the water hyacinth chloroplast; 197, 465 and 1459 ps for the spinach chloroplast, respectively. The slow lifetime fluorescence component is assigned to a collection of associated light harvesting Chi a/b proteins, the fast lifetime component to the reaction center of PS II and the middle lifetime component to the delay fluorescence of recombination of P-680(+) and Pheo(-). The excitation energy conversion efficiency (eta) in PS II RC is defined and calculated on the basis of the 20 ps electron transfer time constant model, 60%, 87% and 91% for the pseudo ginseng, water hyacinth and spinach chloroplasts, respectively. This interesting result is In unconformity with what is assumed to be 100% efficiency in PS II RC. Our result in this work stands in line with the 20 ps electron transfer time constant in PS II rather sound and the water hyacinth plant grows slower than the spinach plant does as envisaged on the efficiency. But, our results predict that those plants can perform highly efficient transfer of photo-excitation energy from the light-harvesting pigment system to the reaction center (closely to 100%). The conclusion contained in this paper reveals the plant growth characteristics expressed in the primary processes of photosynthesis and a relationship between a plant growing rate and its spectroscopy characteristics and fluorescence lifetimes, namely, the slower a plant grows, the less excitation energy conversation efficiency used might be anticipated.
KeywordPseudo Ginseng Water Hyacinth Chloroplast Single Photon Counting Fluorescence Lifetime Excitation Energy Conversation Efficiency
Indexed BySCI
Language英语
WOS IDWOS:000171890600005
PublisherSCIENCE PRESS
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/77092
Collection中国科学院化学研究所
Corresponding AuthorXu, SC
Affiliation1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Xu, SC,Sun, ZY,Ai, XC,et al. Ultrafast spectroscopy studies on the mechanism of electron transfer and energy conversion in the isolated pseudo ginseng, water hyacinth and spinach chloroplasts[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2001,44(4):366-380.
APA Xu, SC.,Sun, ZY.,Ai, XC.,Feng, J.,Zhang, QY.,...&Kuang, TY.(2001).Ultrafast spectroscopy studies on the mechanism of electron transfer and energy conversion in the isolated pseudo ginseng, water hyacinth and spinach chloroplasts.SCIENCE IN CHINA SERIES B-CHEMISTRY,44(4),366-380.
MLA Xu, SC,et al."Ultrafast spectroscopy studies on the mechanism of electron transfer and energy conversion in the isolated pseudo ginseng, water hyacinth and spinach chloroplasts".SCIENCE IN CHINA SERIES B-CHEMISTRY 44.4(2001):366-380.
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