ICCAS OpenIR
High sensitive determination of DNA by use of molecular "light switch" complex of Ru(phen)(2)(dppx)(2+)
Ling, LS; He, ZK; Song, GW; Zeng, YE; Wang, C; Bai, CL; Chen, XD; Shen, P
2001-06-12
Source PublicationANALYTICA CHIMICA ACTA
ISSN0003-2670
Volume436Issue:2Pages:207-214
AbstractA novel fluorimetric method is developed for selective determination of DNA with the molecular "light switch" complex of Ru(phen)(2)(dppx)(2+). The maximal fluorescence intensity is produced in the pH range of 9.3-11.5, with the maximum excitation and emission wavelength of 464 and 594 nm, respectively. Under the optimum conditions, the fluorescence intensity was in proportion to the concentration of DNA. The linear range for calfthymus DNA, salmon sperm DNA and herring sperm DNA is 0-0.2 mug/ml. The limits of detection for calfthymus DNA, salmon sperm DNA and herring sperm DNA are 0.3, 0.24 and 0.74 ng/ml, respectively. And the proposed method was successfully used to determine DNA contents in real sample, which was dealt with RNase before the determination. The mechanism of the fluorescence sensitization is also studied. We also found that Ru(phen)(2)(dppx)(2+) preferalls intercalates into AT base pairs to GC base pairs. (C) 2001 Elsevier Science B.V. All rights reserved.
KeywordRu(Phen)(2)(Dppx)(2+) Dna Fluorimetry Molecular "light Switch"
Indexed BySCI
Language英语
WOS IDWOS:000169276200004
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/76930
Collection中国科学院化学研究所
Corresponding AuthorLing, LS
Affiliation1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
3.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Ling, LS,He, ZK,Song, GW,et al. High sensitive determination of DNA by use of molecular "light switch" complex of Ru(phen)(2)(dppx)(2+)[J]. ANALYTICA CHIMICA ACTA,2001,436(2):207-214.
APA Ling, LS.,He, ZK.,Song, GW.,Zeng, YE.,Wang, C.,...&Shen, P.(2001).High sensitive determination of DNA by use of molecular "light switch" complex of Ru(phen)(2)(dppx)(2+).ANALYTICA CHIMICA ACTA,436(2),207-214.
MLA Ling, LS,et al."High sensitive determination of DNA by use of molecular "light switch" complex of Ru(phen)(2)(dppx)(2+)".ANALYTICA CHIMICA ACTA 436.2(2001):207-214.
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