ICCAS OpenIR
Visualization of the intermediates in a uniform DNA condensation system by tapping mode atomic force microscopy
Liu, D; Wang, C; Lin, Z; Li, JW; Xu, B; Wei, ZQ; Wang, ZG; Bai, CL
2001-08-01
Source PublicationSURFACE AND INTERFACE ANALYSIS
ISSN0142-2421
Volume32Issue:1Pages:15-19
AbstractIn this work, we report observation of the fine structure of cobalt-hexammine-induced DNA condensates by tapping mode atomic force microscopy (AFM). A uniform system with minimum fluctuations of concentration was obtained by introducing a slow-evaporation method, which enables investigation of the intermediate structures in DNA condensation. Some toroid condensates had well-defined small globule subunits; some other condensates were composed of nodular sections with gauze-like complexes spreading around at the outskirts; typical images revealed badly assembled toroidal condensates composed of small globules with a fraction of loosely associated globules dotted in the gauze-like complexes spreading around the badly-assembled part of the toroid. These observations indicate that DNA condensation seems to involve five major stages: initial rapid association of chains to form gauze-like complexes; collapse of these gauze-like complexes at some critical sizes; growth of the critical nucleus by accretion, formation of rods by aggregation of condensed particles; and association of rod-like condensates to form toroids. The results may shed light on uncovering the physical mechanism of DNA condensation by multivalent cations. Copyright (C) 2001 John Wiley & Sons, Ltd.
KeywordAfm Intermediate Dna Cobalt Hexammine Condensation
Indexed ByISTP ; SCI
Language英语
WOS IDWOS:000170551800004
PublisherJOHN WILEY & SONS LTD
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/76748
Collection中国科学院化学研究所
Corresponding AuthorBai, CL
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Liu, D,Wang, C,Lin, Z,et al. Visualization of the intermediates in a uniform DNA condensation system by tapping mode atomic force microscopy[J]. SURFACE AND INTERFACE ANALYSIS,2001,32(1):15-19.
APA Liu, D.,Wang, C.,Lin, Z.,Li, JW.,Xu, B.,...&Bai, CL.(2001).Visualization of the intermediates in a uniform DNA condensation system by tapping mode atomic force microscopy.SURFACE AND INTERFACE ANALYSIS,32(1),15-19.
MLA Liu, D,et al."Visualization of the intermediates in a uniform DNA condensation system by tapping mode atomic force microscopy".SURFACE AND INTERFACE ANALYSIS 32.1(2001):15-19.
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