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| A novel method of capillary electrophoresis for studying the interaction of macromolecule with its small molecule ligand | |
| Fang, M; Zhao, R; Han, HW; Yu, X; Sheng, L; Zang, B; Liu, GQ | |
| 2000-08-20 | |
| Source Publication | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| ISSN | 0253-3820 |
| Volume | 28Issue:8Pages:925-929 |
| Abstract | A novel capillary electrophoresis method for studying the interaction of biomacromolecule with its small molecule ligand has been presented. In the method a home-made capillary electrophoresis set-up coupled with on-line microdialysis by an attached electrode was introduced. The method can be used to determine the free concentration of small molecule without the interference of protein macromolecule. The procedure making of the set-up was described in detail and the sampling mechanism was discussed. As examples the binding constants of sulfamethoxazolum (SMZ) with bovine serum albumin (BSA), BSA with L-tryptophane(Trp) and BSA with D-tryptophane were studied under the physiological conditions (pH 7.4, 50 mmol/L phosphate buffer). The values were 5.8 x 10(4) L/mol, 2.3 x 10(4) L/mol, 1.77 x 10(3) L/mol, respectively. The proposed method is simple and has small sample consuming. It can be used in the desired physiological conditions. |
| Keyword | Affinity Capillary Electrophoresis Affinity Constant On-line Microdialysis Bovine Serum Albumin |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000089026900001 |
| Publisher | FENXI HUAXUE |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.iccas.ac.cn/handle/121111/76550 |
| Collection | 中国科学院化学研究所 |
| Corresponding Author | Fang, M |
| Affiliation | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| Recommended Citation GB/T 7714 | Fang, M,Zhao, R,Han, HW,et al. A novel method of capillary electrophoresis for studying the interaction of macromolecule with its small molecule ligand[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2000,28(8):925-929. |
| APA | Fang, M.,Zhao, R.,Han, HW.,Yu, X.,Sheng, L.,...&Liu, GQ.(2000).A novel method of capillary electrophoresis for studying the interaction of macromolecule with its small molecule ligand.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,28(8),925-929. |
| MLA | Fang, M,et al."A novel method of capillary electrophoresis for studying the interaction of macromolecule with its small molecule ligand".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 28.8(2000):925-929. |
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