ICCAS OpenIR
A novel method of capillary electrophoresis for studying the interaction of macromolecule with its small molecule ligand
Fang, M; Zhao, R; Han, HW; Yu, X; Sheng, L; Zang, B; Liu, GQ
2000-08-20
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume28Issue:8Pages:925-929
AbstractA novel capillary electrophoresis method for studying the interaction of biomacromolecule with its small molecule ligand has been presented. In the method a home-made capillary electrophoresis set-up coupled with on-line microdialysis by an attached electrode was introduced. The method can be used to determine the free concentration of small molecule without the interference of protein macromolecule. The procedure making of the set-up was described in detail and the sampling mechanism was discussed. As examples the binding constants of sulfamethoxazolum (SMZ) with bovine serum albumin (BSA), BSA with L-tryptophane(Trp) and BSA with D-tryptophane were studied under the physiological conditions (pH 7.4, 50 mmol/L phosphate buffer). The values were 5.8 x 10(4) L/mol, 2.3 x 10(4) L/mol, 1.77 x 10(3) L/mol, respectively. The proposed method is simple and has small sample consuming. It can be used in the desired physiological conditions.
KeywordAffinity Capillary Electrophoresis Affinity Constant On-line Microdialysis Bovine Serum Albumin
Indexed BySCI
Language英语
WOS IDWOS:000089026900001
PublisherFENXI HUAXUE
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/76550
Collection中国科学院化学研究所
Corresponding AuthorFang, M
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Fang, M,Zhao, R,Han, HW,et al. A novel method of capillary electrophoresis for studying the interaction of macromolecule with its small molecule ligand[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2000,28(8):925-929.
APA Fang, M.,Zhao, R.,Han, HW.,Yu, X.,Sheng, L.,...&Liu, GQ.(2000).A novel method of capillary electrophoresis for studying the interaction of macromolecule with its small molecule ligand.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,28(8),925-929.
MLA Fang, M,et al."A novel method of capillary electrophoresis for studying the interaction of macromolecule with its small molecule ligand".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 28.8(2000):925-929.
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