ICCAS OpenIR
Atomic force microscopy analysis of intermediates in cobalt hexammine-induced DNA condensation
Liu, D; Wang, C; Li, JW; Lin, Z; Tan, ZK; Bai, CL
2000-08-01
Source PublicationJOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
ISSN0739-1102
Volume18Issue:1Pages:1-9
AbstractThe packaging pathway of cobalt hexammine-induced DNA condensation on the surface of mica was examined by varying the concentration of Co(NH3)(6)(3+) in a dilute DNA solution and visualizing the condensates by atomic force microscopy (AFM). Images reveal that cobalt hexammine-induced DNA condensation on mica involves well-defined structures. At 30 mu M Co(NH3)(6)(3+), prolate ellipsoid condensates composed of relatively shorter rods with linkages between them are: formed. At 80 mu M Co(NH3)(6)(3+), the condensed features include toroids with average diameter of similar to 240 nm as well as U-shaped and rod-like condensates with nodular appearances. The results imply that the condensates, whether toroids, U-shaped or rod-like structures have similar intermediate state which includes relatively shorter rod-like segments. The average size: of the condensed toroids after incubated at room temperature for 5 h (similar to 240 nm) is much larger than that incubated for 0.5 h (similar to 100 nm). The results indicate that the condensation of DNA by Co(NH3)(6)(3+) is a kinetic-controlled process.
Indexed BySCI
Language英语
WOS IDWOS:000089577200002
PublisherADENINE PRESS INC
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/76538
Collection中国科学院化学研究所
Corresponding AuthorLiu, D
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Liu, D,Wang, C,Li, JW,et al. Atomic force microscopy analysis of intermediates in cobalt hexammine-induced DNA condensation[J]. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS,2000,18(1):1-9.
APA Liu, D,Wang, C,Li, JW,Lin, Z,Tan, ZK,&Bai, CL.(2000).Atomic force microscopy analysis of intermediates in cobalt hexammine-induced DNA condensation.JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS,18(1),1-9.
MLA Liu, D,et al."Atomic force microscopy analysis of intermediates in cobalt hexammine-induced DNA condensation".JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS 18.1(2000):1-9.
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