ICCAS OpenIR
Photosensitized formation of singlet oxygen by phycobiliproteins in neutral aqueous solutions
Zhang, SP; Zhao, JQ; Jiang, LJ
2000
Source PublicationFREE RADICAL RESEARCH
ISSN1071-5762
Volume33Issue:5Pages:489-496
AbstractPhycobiliproteins (PBPs) are a type of promising sensitizers for photodynamic therapy (PDT). Upon irradiation (lambda >500nm) of an oxygen-saturated aqueous solution of phycobiliproteins, particularly, C-phycocyanin (C-PC), allophycocyanin (APC) or R-phycoerythrin (R-PE), the formation of singlet oxygen (O-1(2)) was detected by using imidazole in the presence of p-nitrosodimethylaniline (RNO). The bleaching of RNO caused by the presence of imidazole in our system showed typical concentration dependence with a maximum at about 8mM imidazole, which is in agreement with the formation of O-1(2) in addition, the generation of O-1(2) was verified further in the presence of D2O and specific singlet oxygen quencher - 1,4-diazabicyclo [2,2,2] octane (DABCO) and sodium azide (NaN3). Our experimental results indicated that APC possesses high ability to generate reactive oxygen species and the relative quantum yields of photogeneration of O-1(2) by PBPs are as follows: APC > C-PC > R-PE.
KeywordPhycobiliprotein Singlet Oxygen Photobleaching
Indexed BySCI
Language英语
WOS IDWOS:000166441800005
PublisherHARWOOD ACAD PUBL GMBH
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/76442
Collection中国科学院化学研究所
Corresponding AuthorZhao, JQ
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, SP,Zhao, JQ,Jiang, LJ. Photosensitized formation of singlet oxygen by phycobiliproteins in neutral aqueous solutions[J]. FREE RADICAL RESEARCH,2000,33(5):489-496.
APA Zhang, SP,Zhao, JQ,&Jiang, LJ.(2000).Photosensitized formation of singlet oxygen by phycobiliproteins in neutral aqueous solutions.FREE RADICAL RESEARCH,33(5),489-496.
MLA Zhang, SP,et al."Photosensitized formation of singlet oxygen by phycobiliproteins in neutral aqueous solutions".FREE RADICAL RESEARCH 33.5(2000):489-496.
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