ICCAS OpenIR
Monosized non-porous poly(glycidyl methacrylate) beads as the support for high performance affinity chromatography
Yu, X; Zhao, R; Fang, M; Xiong, SX; Guo, YH; Su, TS; Liu, GQ
2000-05-20
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume28Issue:5Pages:544-548
AbstractA new synthetic support, monosized, non-porous, cross-linked bead CL-poly(glycidyl methacrylate) (CL-PGMA) containing active epoxide groups was synthesized and used as the support of affinity chromatography. The concentration of the epoxide groups on the surface of CL-PGMA beads was measured and the non-specific adsorption to protein was characterized. Trypsin inhibitor was chosen as the ligand. The trypsin was purified 2.5 times by using the trypsin inhibitor immobilized CL-PGMA affinity column. The chromatographic characterization was shown that the CL-PGMA bead was very suitable for the high performance affinity chromatography. The effective elimination of non-specific adsorption was carried out by adding 1% acetonitrile in mobile phase. The recovery of human serum albumin was more than 99%.
KeywordHigh Performance Affinity Chromatography Monosized Non-porous Poly(Glycidyl Methacrylate) Beads Trypsin Inhibitor
Indexed BySCI
Language英语
WOS IDWOS:000087271600004
PublisherFENXI HUAXUE
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/76124
Collection中国科学院化学研究所
Corresponding AuthorZhao, R
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yu, X,Zhao, R,Fang, M,et al. Monosized non-porous poly(glycidyl methacrylate) beads as the support for high performance affinity chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2000,28(5):544-548.
APA Yu, X.,Zhao, R.,Fang, M.,Xiong, SX.,Guo, YH.,...&Liu, GQ.(2000).Monosized non-porous poly(glycidyl methacrylate) beads as the support for high performance affinity chromatography.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,28(5),544-548.
MLA Yu, X,et al."Monosized non-porous poly(glycidyl methacrylate) beads as the support for high performance affinity chromatography".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 28.5(2000):544-548.
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