ICCAS OpenIR
Effect of CO2 and CHF3 on the solubilization of protein in reverse micelles
Zhang, HF; Han, BX; Yang, GY; Yan, HK
2000-12-15
Source PublicationJOURNAL OF COLLOID AND INTERFACE SCIENCE
ISSN0021-9797
Volume232Issue:2Pages:269-272
AbstractThe effect of dissolved CO2 and CHF3 on the precipitation of bovine serum albumin (BSA) from reverse micelles of sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in 2,2,4-trimethylpentane (isooctane) has been studied at 308.15 K. It was found that BSA can be precipitated completely by CO2 while the AOT and water remain in the iso-octane continuous phase. This opens up a possibility for recovery of protein solids directly from reverse micellar solutions. CHF3 is also an effective agent for precipitation of BSA in the reverse micelles when the expansion coefficient of CHF3 exceeds 0.4. However, the condition required for complete removal of the BSA using CHF3 results in some precipitation of AOT and water from the solution. (C) 2000 Academic Press.
KeywordCompressed Gases Reverse Micelles Protein Precipitation Co2 Chf3
DOI10.1006/jcis.2000.7214
Indexed BySCI
Language英语
WOS IDWOS:000165866300008
PublisherACADEMIC PRESS INC
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/76104
Collection中国科学院化学研究所
Corresponding AuthorHan, BX
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, HF,Han, BX,Yang, GY,et al. Effect of CO2 and CHF3 on the solubilization of protein in reverse micelles[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE,2000,232(2):269-272.
APA Zhang, HF,Han, BX,Yang, GY,&Yan, HK.(2000).Effect of CO2 and CHF3 on the solubilization of protein in reverse micelles.JOURNAL OF COLLOID AND INTERFACE SCIENCE,232(2),269-272.
MLA Zhang, HF,et al."Effect of CO2 and CHF3 on the solubilization of protein in reverse micelles".JOURNAL OF COLLOID AND INTERFACE SCIENCE 232.2(2000):269-272.
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