ICCAS OpenIR
Affinity chromatography based on the oligohistines as the ligands
Yu, X; Zhao, R; Fang, M; Xiong, SX; Su, TS; Liu, GQ
2000-04-01
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN0251-0790
Volume21Issue:4Pages:532-534
AbstractA series of affinity media were synthesized by using the oligohistines, His-Gly, (His)(3)-Gly and (His)(5)-Gly as the ligands and the home-made monosized, non-porous copolymer (CL-PGMA) beads containing active epoxide groups as the support. The affinity chromatographic behavior between IgG and the oligohistines was investigated at pH 3. 0, 4. 5, 7. 0 and 10. 0. It showed the specific interaction at pH 4. 5. The dissociation constants of IgG and oligohistine media (1H, 3H, 5H) were 3. 037 X 10(-6), 2. 459 X 10(-6) and 1. 905 X 10(-6) L/mol, respectively. The dissociation constants decreased with increasing the number of histine-residue in oligopeptide chains.
KeywordIgg Oligohistine Hpac Dissociation Constant Synthesis Of Peptide In Solid Phase
Indexed BySCI
Language英语
WOS IDWOS:000086574700009
PublisherHIGHER EDUCATION PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/75870
Collection中国科学院化学研究所
Corresponding AuthorZhao, R
AffiliationChinese Acad Sci, Ctr Mol Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yu, X,Zhao, R,Fang, M,et al. Affinity chromatography based on the oligohistines as the ligands[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2000,21(4):532-534.
APA Yu, X,Zhao, R,Fang, M,Xiong, SX,Su, TS,&Liu, GQ.(2000).Affinity chromatography based on the oligohistines as the ligands.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,21(4),532-534.
MLA Yu, X,et al."Affinity chromatography based on the oligohistines as the ligands".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 21.4(2000):532-534.
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