Preparation and characterization of monosized cross-linked PMMA resins with hydrophilic surface
Guo, YH; Yang, YG; Su, TS
AbstractThis paper presents the novel monosized resins with hydrophilic surface coated polysaccharides, including synthesis of the matrix beads,modification of the particle surface and characterization of HPLC properties of the modified products. The monosized matrix beads based on poly(methyl methacrylate-co-divinylbenzene) (PMMA-DVB) were synthesized by seed swelling polymerization. The linear polystyrene particles as seeds were swollen by an emulsifying mixture of monomer, cross-linker and initiator in the aqueous solution containing stabilizer at the room temperature, then the swelling products were polymerized in the same solution system at 70 degrees C, finally the polymerization products were fully washed with water. The particle parameters and apparent morphology of the matrix beads were characterized by scanning electron microscopy (SEM). The results show that the polymerization method is suitable to the preparation of monosized PMMA-DVB beads with particle diameters of 5 similar to 15 mu m, the particle dispersion coefficient of the polymerization products with different cross-linking densities is less than 2%. After being hydrolyzed with a 30% (W/V) NaOH solution at 80 degrees C, the PMMA-DVB beads were transformed into the hydrolyzed resins containing carboxylic groups on the particle surface. Dextran and DEAE-Dextran were coated respectively by the impregnation method, and then were cross-linked respectively with 1,4-butanedol diglycidyl ether as cross-linker. Two new modified resins with covering layer of cross-linked polysaccharides on the particle surface were obtained. The polysaccharides content of the modified resins was about 7% (W/W) of the matrix beads. In order to get a uniform covering layer, the coating and cross-linking process was generally divided into two or three times to accomplish with same operation method. SEM observation showed that the physical morphology of the two modified resins still kept monodisperse particle as matrix beads. Owing to highly cross-linking network structure, the modified resins exhibit good physical and chemical stability. The particles can withstand high pressure operation, can be treated with acidic and alkaline solutions (pH = 1 similar to 14). Monodisperse particle size,good particle rigidity and the surface coated by polysaccharides make the modified resins as HPLC packings possessing high column efficiency, high permeability and excellent hydrophilic properties. The experiments show that on chromatographic columns packed with the two modified resins, human serum albumin possesses good elution behavior in elution system of phosphate buffer (pH = 3.8),the protein recovery is over 97% and 99% respectively.
KeywordMonosized Beads Resin With Hydrophilic Surface Hplc Packings
Indexed BySCI
WOS IDWOS:000089889100025
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Document Type期刊论文
Corresponding AuthorGuo, YH
AffiliationChinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Guo, YH,Yang, YG,Su, TS. Preparation and characterization of monosized cross-linked PMMA resins with hydrophilic surface[J]. ACTA POLYMERICA SINICA,2000(5):641-643.
APA Guo, YH,Yang, YG,&Su, TS.(2000).Preparation and characterization of monosized cross-linked PMMA resins with hydrophilic surface.ACTA POLYMERICA SINICA(5),641-643.
MLA Guo, YH,et al."Preparation and characterization of monosized cross-linked PMMA resins with hydrophilic surface".ACTA POLYMERICA SINICA .5(2000):641-643.
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