ICCAS OpenIR
pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films
Jussila, Tapio1; Li, Meiling2; Tkachenko, Nikolai V.1; Parkkinen, Sinikka3; Li, Baofang2; Jiang, Long2; Lemmetyinen, Helge1
2000
Source PublicationINTERNATIONAL JOURNAL OF PHOTOENERGY
ISSN1110-662X
Volume2Pages:41-45
AbstractBacteriorhodopsin (bR) multilayer films were successfully prepared by alternating depositions of cationic poly(diallyldimethylammonium chloride) (PDAC) polyelectrolyte and purple membrane (PM). The advantage of the method when compared to other thin film techniques is its simplicity to prepare large self-assembled films. The orientations of the bR molecules in the films were studied by a transient photovoltage method. The polarity of the photoresponse signals showed that in basic conditions the cytoplasmic side of PM was adsorbed onto the polyelectrolyte layer, whereas in acidic conditions the extracellular side was adsorbed onto the polyelectrolyte layer. The photocycle of the bR in the films was studied by using the conventional flash photolysis method.
Indexed BySCI
Language英语
WOS IDWOS:000208867600006
PublisherHINDAWI PUBLISHING CORPORATION
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/75612
Collection中国科学院化学研究所
Corresponding AuthorJussila, Tapio
Affiliation1.Tampere Univ Technol, Inst Mat Chem, FIN-33101 Tampere, Finland
2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Lab Colloid & Interface Sci, Beijing 100101, Peoples R China
3.Univ Joensuu, Dept Biol, FIN-80101 Joensuu, Finland
Recommended Citation
GB/T 7714
Jussila, Tapio,Li, Meiling,Tkachenko, Nikolai V.,et al. pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films[J]. INTERNATIONAL JOURNAL OF PHOTOENERGY,2000,2:41-45.
APA Jussila, Tapio.,Li, Meiling.,Tkachenko, Nikolai V..,Parkkinen, Sinikka.,Li, Baofang.,...&Lemmetyinen, Helge.(2000).pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films.INTERNATIONAL JOURNAL OF PHOTOENERGY,2,41-45.
MLA Jussila, Tapio,et al."pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films".INTERNATIONAL JOURNAL OF PHOTOENERGY 2(2000):41-45.
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