ICCAS OpenIR
Characterization of the transient species generated by the photoexcitation of C-phycocyanin from Spirulina platensis: a laser photolysis and pulse radiolysis study
Zhang, SP; Qian, SP; Zhao, JQ; Yao, SD; Jiang, LJ
1999-10-18
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
ISSN0304-4165
Volume1472Issue:1-2Pages:270-278
AbstractNanosecond laser flash photolysis and pulse radiolysis were used to generate and characterize the triplet state and cation radical of C-phycocyanin (C-PC) from Spirulina platensis. The transient absorption spectra of C-PC were measured from direct excitation and acetone sensitization in aqueous solution at room temperature by KrF (248 nm) laser flash photolysis. Laser-induced transient species have been characterized by the method of acetone sensitization and one-electron oxidation. In nitrous oxide-saturated phosphate buffer saline (pH = 7.0) of C-PC, the produced intermediates are assigned to the excited triplet state and the radical cation. Using acetone as photosensitizer, the C-PC excited triplet states produced via triplet-triplet energy transfer and the C-PC radical cation from electron transfer reaction were further confirmed. Furthermore, the corresponding kinetic parameters were determined. To our knowledge, the transient absorption spectra of C-PC have been reported for the first time. (C) 1999 Elsevier Science B.V. All rights reserved.
KeywordC-phycocyanin Triplet State Radical Cation Laser Photolysis Pulse
Indexed BySCI
Language英语
WOS IDWOS:000083347800030
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/75574
Collection中国科学院化学研究所
Corresponding AuthorZhao, JQ
Affiliation1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
2.Shanghai Inst Nucl Res, Chinese Acad Sci, Lab Radiat Chem, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Zhang, SP,Qian, SP,Zhao, JQ,et al. Characterization of the transient species generated by the photoexcitation of C-phycocyanin from Spirulina platensis: a laser photolysis and pulse radiolysis study[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1999,1472(1-2):270-278.
APA Zhang, SP,Qian, SP,Zhao, JQ,Yao, SD,&Jiang, LJ.(1999).Characterization of the transient species generated by the photoexcitation of C-phycocyanin from Spirulina platensis: a laser photolysis and pulse radiolysis study.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1472(1-2),270-278.
MLA Zhang, SP,et al."Characterization of the transient species generated by the photoexcitation of C-phycocyanin from Spirulina platensis: a laser photolysis and pulse radiolysis study".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1472.1-2(1999):270-278.
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