ICCAS OpenIR
Layer-by-layer assembly of DNA films and their interactions with dyes
Lang, J; Lin, MH
1999-12-23
Source PublicationJOURNAL OF PHYSICAL CHEMISTRY B
ISSN1089-5647
Volume103Issue:51Pages:11393-11397
AbstractLayer-by-layer assembly of DNA film was fabricated on quartz plates through alternative adsorption with poly(allylamine) in order to investigate the interaction of DNA film with dyes. The film was water-insoluble and can interact with many water-soluble dyes. It has been found that ethidium bromide (EB) and 5,10,15,20-tetrakis(4-N-methylpyridyl)porphine tetra(p-toluenesulfonate) (TMPyP) can intercalate into the DNA film, which has been verified from the UV and fluorescence :spectra and circular dichroism (CD) measurements. Acridine orange (AO) can also interact with the DNA film, but it seems that AO attaches to the DNA as a dimer. It has been further confirmed that TMPyP can predominantly intercalate into the assembled DNA film, while all the dyes coexisted in the aqueous solution. In the case of TMPyP solution only, TMPyP intercalated predominantly into the G-C site, while in the mixture it went mainly into the A-T site of the DNA film.
Indexed BySCI
Language英语
WOS IDWOS:000084601400022
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:89[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/75194
Collection中国科学院化学研究所
Corresponding AuthorLin, MH
AffiliationChinese Acad Sci, Lab Colloid & Interface Sci, Ctr Mol Sci, Inst Chem, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Lang, J,Lin, MH. Layer-by-layer assembly of DNA films and their interactions with dyes[J]. JOURNAL OF PHYSICAL CHEMISTRY B,1999,103(51):11393-11397.
APA Lang, J,&Lin, MH.(1999).Layer-by-layer assembly of DNA films and their interactions with dyes.JOURNAL OF PHYSICAL CHEMISTRY B,103(51),11393-11397.
MLA Lang, J,et al."Layer-by-layer assembly of DNA films and their interactions with dyes".JOURNAL OF PHYSICAL CHEMISTRY B 103.51(1999):11393-11397.
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