ICCAS OpenIR
Investigation on process parameters involved in preparation of poly-DL-lactide-poly(ethylene glycol) microspheres containing Leptospira Interrogans antigens
Li, XH; Deng, XM; Yuan, ML; Xiong, CD; Huang, ZT; Zhang, YH; Jia, WX
1999-02-15
Source PublicationINTERNATIONAL JOURNAL OF PHARMACEUTICS
ISSN0378-5173
Volume178Issue:2Pages:245-255
AbstractBlock copolymer, poly-DL-lactide-poly(ethylene glycol) (PELA) with 11.5% of poly(ethylene glycol) (PEG) content was prepared by bulk ring-opening polymerization using stannous chloride as initiator. PELA microspheres with entrapped Leptospira Interrogans antigens, outer membrane protein (OMP) were elaborated by solvent extraction method based on the formation of multiple w/o/w emulsion, and the resulting microspheres were characterized with respect to particle size, OMP entrapment and morphology characteristics. The purpose of the present study is to perform the optimization of preparative parameters for OMP-loaded PELA micropsheres to control particle size and improve the OMP encapsulation efficiency. Of all the parameters investigated, the polymer concentration of organic phase and the external aqueous phase volume play major roles on particle size, while the organic phase volume, internal aqueous phase volume and the addition of surfactant into the internal aqueous phase display considerable effects on OMP loading efficiency. A small volume of internal aqueous phase and intermediate volumes of organic phase and external aqueous phase were favorable to achieve micropsheres with a size of 1-2 mu m and high antigen encapsulation efficiency (70-80%). In vitro OMP release profiles from PELA microspheres consist of a small burst release followed by a gradual release phase. The OMP release rate shows some relations with the porous and water-swollen inner structure of the microspheres matrix. The presence of surfactant in microspheres accelerates OMP release, but the OMP entrapment within microspheres shows limited effects on the release profile. (C) 1999 Elsevier Science B.V. All rights reserved.
KeywordPoly-dl-lactide-poly(Ethylene Glycol) Outer Membrane Protein Preparative Parameter Encapsulation Efficiency In Vitro Release
Indexed BySCI
Language英语
WOS IDWOS:000078849000010
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:73[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/75110
Collection中国科学院化学研究所
Corresponding AuthorLi, XH
Affiliation1.Chinese Acad Sci, Chengdu Inst Organ Chem, Chengdu 610041, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
3.W China Univ Med Sci, Chengdu 610041, Peoples R China
Recommended Citation
GB/T 7714
Li, XH,Deng, XM,Yuan, ML,et al. Investigation on process parameters involved in preparation of poly-DL-lactide-poly(ethylene glycol) microspheres containing Leptospira Interrogans antigens[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,1999,178(2):245-255.
APA Li, XH.,Deng, XM.,Yuan, ML.,Xiong, CD.,Huang, ZT.,...&Jia, WX.(1999).Investigation on process parameters involved in preparation of poly-DL-lactide-poly(ethylene glycol) microspheres containing Leptospira Interrogans antigens.INTERNATIONAL JOURNAL OF PHARMACEUTICS,178(2),245-255.
MLA Li, XH,et al."Investigation on process parameters involved in preparation of poly-DL-lactide-poly(ethylene glycol) microspheres containing Leptospira Interrogans antigens".INTERNATIONAL JOURNAL OF PHARMACEUTICS 178.2(1999):245-255.
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