ICCAS OpenIR
Effects of training of operant conditioned reflex on free amino acids in hippocampus of rats
Shu, HJ; Han, HW; Zhao, R; Chen, Y; Liu, GQ
1999-11-01
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN0251-0790
Volume20Issue:11Pages:1703-1707
AbstractChanges of intracellular levels of free amino acids(FAA) in hippocampus of freely moving rats were measured using intracerebral microdialysis coupled to Orthophthaladehyde (OPA) pre-column derivatization-reversed phase HPLC-fluorescence detection, The analytical method was systematically optimized and the real samples were determined. It was employed to analyze the trace level of free amino acids(FAA) and possible to qualitative and quantitative 18 kinds of amino acids in every 5 mu L microdialysate. The sensitivity and the linear relation between the amounts and the peak areas were satisfied. The recoveries were 82%-109%, The detection limits reached 0. 5-5. 7 pmol for each FAA. A significant decrease of 14 FAA after training of OCR was founded in comparison to basal condition(before training), In fore finds of neurotransmitter amino acids(NAA), excitatory NAA, glutamic acid and aspartic acid increased 19. 12% and 53. 40% respectively than before training, Inhibitory NAA, gamma-aminobutyric (GABA), increased 138. 17% than before training. The results confirm that metabolic increase of FAA in hippocampus can be induced by learning and memory in rats, The intracerebral microdialysis technique thus appears to be a useful tool to monitor changes in physiological activity of learning and memory during ongoing behavior.
KeywordOperant Conditioned Reflex Hippocampus Learning Amino Acid Analysis
Indexed BySCI
Language英语
WOS IDWOS:000083723600010
PublisherHIGHER EDUCATION PRESS
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/75086
Collection中国科学院化学研究所
Corresponding AuthorHan, HW
AffiliationChinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Shu, HJ,Han, HW,Zhao, R,et al. Effects of training of operant conditioned reflex on free amino acids in hippocampus of rats[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,1999,20(11):1703-1707.
APA Shu, HJ,Han, HW,Zhao, R,Chen, Y,&Liu, GQ.(1999).Effects of training of operant conditioned reflex on free amino acids in hippocampus of rats.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,20(11),1703-1707.
MLA Shu, HJ,et al."Effects of training of operant conditioned reflex on free amino acids in hippocampus of rats".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 20.11(1999):1703-1707.
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