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Force spectroscopy between acetylcholinesterase molecule and its natural substrate to study the effects of inhibitors and reactivators on enzyme activity
Yingge, Z; Delu, Z; Chunli, B; Chen, W
1999-10-15
Source PublicationLIFE SCIENCES
ISSN0024-3205
Volume65Issue:21Pages:PL253-PL260
AbstractThe force spectrum (FS) between acetylcholinesterase (AChE) molecule and its natural substrates acetylcholine (ACh) and the influences of AChE inhibitors and reactivators have been investigated with atomic force microscopy (AFM) at single molecule level in real-time. AChE and ACh were covalently immobilized onto the surfaces of gold-plated mica and Si3N4 tip of the atomic force microscope respectively. First, AChE was imaged in image mode of AFM and one of AChE molecules was selected as the center of the scanning. Then scanning mode was changed into force scanning mode and FS was recorded in a frequency of 5.s(-1). Solutions of drugs or toxicants can be injected from the fluid-in tube of the fluid cell at any desired time. The FS between ideally immobilized normal AChE, Inhibited AChE or aged AChE and ACh each had their own shape features. The influences of drugs or toxicants on these features could be observed in real-time on the screen of the computer. These results demonstrated that AFM force spectroscopy could be used as a new method to study the effects of drugs and toxicants on the activity of the enzyme in pharmacology and toxicology. (C) 1999 Elsevier Science Inc.
KeywordForce Spectroscopy Atomic Force Microscopy Acetylcholinesterases Acetylcholine
Indexed BySCI
Language英语
WOS IDWOS:000083218400014
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/75070
Collection中国科学院化学研究所
Corresponding AuthorYingge, Z
Affiliation1.Acad Mil Med Sci, Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yingge, Z,Delu, Z,Chunli, B,et al. Force spectroscopy between acetylcholinesterase molecule and its natural substrate to study the effects of inhibitors and reactivators on enzyme activity[J]. LIFE SCIENCES,1999,65(21):PL253-PL260.
APA Yingge, Z,Delu, Z,Chunli, B,&Chen, W.(1999).Force spectroscopy between acetylcholinesterase molecule and its natural substrate to study the effects of inhibitors and reactivators on enzyme activity.LIFE SCIENCES,65(21),PL253-PL260.
MLA Yingge, Z,et al."Force spectroscopy between acetylcholinesterase molecule and its natural substrate to study the effects of inhibitors and reactivators on enzyme activity".LIFE SCIENCES 65.21(1999):PL253-PL260.
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