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A novel method for studying the interaction of macromolecule with small molecule by means of affinity capillary electrophoresis
Fang, M; Sheng, L; Han, HW; Yu, X; Zhao, R; Liu, GQ
1999-03-01
Source PublicationCHINESE CHEMICAL LETTERS
ISSN1001-8417
Volume10Issue:3Pages:235-238
AbstractA novel capillary electrophoresis method coupled with on-line microdialysis using an attachable electrode has been developed to study the interaction of macromolecule with small molecule. The binding constants of bovine serum album (BSA) with D,L-tryptophan (Trp), sulfamethoxazole (SMZ) with trypsin and chymotrypsin were determined. These values are 2.3 x 10(4)L/mol for BSA-L-Trp; 1.77 x 10(3) L/mol for BSA-D-Trp in pH 7.4, 50 mmol/ L phosphate; 1.4 x 10(4) L/mol for SMZ- trypsin and 6.0 x 10(3) L/mol for SMZ-chymotrypsin in pH 6.5, 25 mmol/L Tris buffer. The proposed method has merits of speed, low sample consumption and readily available to be performed in desired physiological conditions.
KeywordInteraction Of MacroMolecule With Small Molecule Binding Constant Affinity Capillary Electrophoresis Microdialysis Membrane
Indexed BySCI
Language英语
WOS IDWOS:000081029600016
PublisherCHINESE CHEMICAL SOCIETY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/75008
Collection中国科学院化学研究所
AffiliationChinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Fang, M,Sheng, L,Han, HW,et al. A novel method for studying the interaction of macromolecule with small molecule by means of affinity capillary electrophoresis[J]. CHINESE CHEMICAL LETTERS,1999,10(3):235-238.
APA Fang, M,Sheng, L,Han, HW,Yu, X,Zhao, R,&Liu, GQ.(1999).A novel method for studying the interaction of macromolecule with small molecule by means of affinity capillary electrophoresis.CHINESE CHEMICAL LETTERS,10(3),235-238.
MLA Fang, M,et al."A novel method for studying the interaction of macromolecule with small molecule by means of affinity capillary electrophoresis".CHINESE CHEMICAL LETTERS 10.3(1999):235-238.
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