ICCAS OpenIR
The folding and enzymatic activity of glucose oxidase in the glycolipid matrixes of different charges
Li, JR; Du, YK; Boullanger, P; Jiang, L
1999-09-08
Source PublicationTHIN SOLID FILMS
ISSN0040-6090
Volume352Issue:1-2Pages:213-217
AbstractThe influence of lipid matrixes with different charges on the protein folding behavior has been investigated by using the Langmuir-Blodgett (LB) technique. To understand the conformation change under different forces, Glucose oxidase (GOD) from Aspergilius Niger was used as a protein model. Eight glycolipids(1,2-o-dialkyl-3-o-beta-D-glycosylglycerols and alkyl 2-amino-2-deoxy-beta-D-glucopyranoside) were used as the matrixes for this investigation. It was observed that the GOD can penetrate into neutral glycolipid monolayer and change its conformation in favor of the alpha-helix formation. Moreover, GOD strongly adsorbed to the positive charged glycolipid monolayer and change its conformation in favor of the beta-sheet formation. Enzymatic activity measurements showed that the more the alpha-helix conformation content is in the GOD, the higher activity the GOD will be. This fact suggested a new way to mediate the conformation of protein in organized molecular assemblies, and provided a new thinking for the preparation of biomimetic film and biosensor. (C) 1999 Elsevier Science S.A. All rights reserved.
KeywordBiomaterials Monolayers Multilayers Surface Structure
Indexed BySCI
Language英语
WOS IDWOS:000082709600034
PublisherELSEVIER SCIENCE SA
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/74814
Collection中国科学院化学研究所
Corresponding AuthorJiang, L
Affiliation1.Acad Sinica, Inst Photog Chem, Ctr Mol Sci, Lab Colloid & Interface, Beijing 100101, Peoples R China
2.Univ Lyon 1, Lab Chim Organ 2, CPE Lyon, F-69622 Villeurbanne, France
Recommended Citation
GB/T 7714
Li, JR,Du, YK,Boullanger, P,et al. The folding and enzymatic activity of glucose oxidase in the glycolipid matrixes of different charges[J]. THIN SOLID FILMS,1999,352(1-2):213-217.
APA Li, JR,Du, YK,Boullanger, P,&Jiang, L.(1999).The folding and enzymatic activity of glucose oxidase in the glycolipid matrixes of different charges.THIN SOLID FILMS,352(1-2),213-217.
MLA Li, JR,et al."The folding and enzymatic activity of glucose oxidase in the glycolipid matrixes of different charges".THIN SOLID FILMS 352.1-2(1999):213-217.
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