ICCAS OpenIR
Selection of affinity ligands for protein purification from proteolytic digests
Yang, YH; Chase, HA; Liu, GQ
1998-03-01
Source PublicationBIOTECHNOLOGY TECHNIQUES
ISSN0951-208X
Volume12Issue:3Pages:245-251
AbstractA simple method for the selection of affinity ligands from proteolytic digests by affinity chromatography is proposed. A small proportion of the peptides in the trypsin digest of bovine serum albumin (BSA) or the pepsin digest of cytochrome are retarded on insulin-immobilised or HSA (human serum albumin)-immobilised affinity columns, respectively. The peptides in these selected fractions can be immobilised onto solid phases and used in affinity chromatography procedures for the purification of insulin or HSA.
Indexed BySCI
Language英语
WOS IDWOS:000072477800013
PublisherKLUWER ACADEMIC PUBL
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/74444
Collection中国科学院化学研究所
Corresponding AuthorChase, HA
Affiliation1.Univ Cambridge, Dept Chem Engn, Cambridge CB2 3RA, England
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yang, YH,Chase, HA,Liu, GQ. Selection of affinity ligands for protein purification from proteolytic digests[J]. BIOTECHNOLOGY TECHNIQUES,1998,12(3):245-251.
APA Yang, YH,Chase, HA,&Liu, GQ.(1998).Selection of affinity ligands for protein purification from proteolytic digests.BIOTECHNOLOGY TECHNIQUES,12(3),245-251.
MLA Yang, YH,et al."Selection of affinity ligands for protein purification from proteolytic digests".BIOTECHNOLOGY TECHNIQUES 12.3(1998):245-251.
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