Formation and characteristics of an unusual lambda-DNA species
Cao, EH; Sun, XG; Bai, CL; Liu, CQ; He, YJ; Wang, XW; Fang, Y
AbstractAn unusual DNA species, termed as DNA species A, has been isolated and purified from thermal-denatured lambda-DNA Hind III by Sephadex G-200 gel filtration. Our studies indicate that DNA species A is resistant to DNase I digestion and has a higher melting point. The new DNA species showed a lower absorbency at 260 nm, and a lower fluorescence quantum yield after interaction with ethidium bromide (EB) than native double-stranded lambda-DNA. CD spectrum of DNA species A consists of a broad positive band centered at 245 nm and a weak negative band at 220 nm. transmission electron microscope (TEM) visualizations showed that their lengths of DNA species A fell mainly in three regions (300-500 nm, 750-1000 nm and 1500 nm) that corresponded to three fluorescence bands in the EB-stained gels. Their apparent width and height were 65-75 nm and 2.2 nm respectively as observed by images of atomic force microscope (AFM).
KeywordLambda-dna Triple-stranded Dna Melting Temperature Cd Spectra Fluorescence Spectrum Transmission Electron Microscope Atomic Force Microscope
Indexed BySCI
WOS IDWOS:000073191000009
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Document Type期刊论文
Corresponding AuthorCao, EH
Affiliation1.Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China
2.Acad Sinica, Inst Chem, Beijing 100080, Peoples R China
3.Acad Sinica, Inst Zool, Kunming 650223, Peoples R China
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GB/T 7714
Cao, EH,Sun, XG,Bai, CL,et al. Formation and characteristics of an unusual lambda-DNA species[J]. BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL,1997,43(3):541-550.
APA Cao, EH.,Sun, XG.,Bai, CL.,Liu, CQ.,He, YJ.,...&Fang, Y.(1997).Formation and characteristics of an unusual lambda-DNA species.BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL,43(3),541-550.
MLA Cao, EH,et al."Formation and characteristics of an unusual lambda-DNA species".BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL 43.3(1997):541-550.
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