ICCAS OpenIR
Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization
Dong, Xiaoqing1,3; Wang, Yu1; Huang, Yubin1; Liu, Junqiu2; Jing, Xiabin1
2011
Source PublicationJOURNAL OF MATERIALS CHEMISTRY
ISSN0959-9428
Volume21Issue:40Pages:16147-16152
AbstractWell-defined porous dextrans bound with glutathione (GSH) were prepared in four steps: (1) vinylation of dextran by reacting it with glycidyl methacrylate (GMA), (2) preparation of the aqueous phase with vinylated dextran, surfactant and reduced GSH, (3) HIPE polymerization of vinylated dextran initiated by AIBN in an oil phase and incorporating GSH onto the dextran chains by chain transfer reactions, and (4) removal of the oil phase and water soluble impurities. These porous materials had interconnected pores, very thin walls and enough GSH moieties for glutathione S-transferase (GST) separation via specific GSH-GST binding. The representative material prepared was filled in the column for separation of the GST from the crude cell lysate. The result shows that this material has a very high selectivity for GST separation.
DOI10.1039/c1jm12567b
Indexed BySCI
Language英语
WOS IDWOS:000295580800042
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/73796
Collection中国科学院化学研究所
Corresponding AuthorJing, Xiabin
Affiliation1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
2.Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Dong, Xiaoqing,Wang, Yu,Huang, Yubin,et al. Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization[J]. JOURNAL OF MATERIALS CHEMISTRY,2011,21(40):16147-16152.
APA Dong, Xiaoqing,Wang, Yu,Huang, Yubin,Liu, Junqiu,&Jing, Xiabin.(2011).Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization.JOURNAL OF MATERIALS CHEMISTRY,21(40),16147-16152.
MLA Dong, Xiaoqing,et al."Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization".JOURNAL OF MATERIALS CHEMISTRY 21.40(2011):16147-16152.
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