ICCAS OpenIR
Discrimination of the false-positive signals of molecular beacons by combination of heat inactivation and using single walled carbon nanotubes
Su, Xin; Zhang, Chen; Zhao, Meiping
2011-04-15
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume26Issue:8Pages:3596-3601
AbstractMolecular beacons (MBs) have been extensively used for real-time monitoring of RNA/DNA and protein molecules. However, such versatility also brings about multiple sources of positive signals. Moreover, the covalently attached quencher or fluorophore may even be cleaved from the strand by the exonucleases, followed by complete degradation of the probe. These undesirable false-positive signals (FPSs) have seriously limited the application of MBs to detect real world samples. In this paper, we propose a novel and efficient approach for discrimination of FPSs of MBs due to non-specific MB-protein interactions and nuclease degradation by combination of heat inactivation and using single walled carbon nanotubes (SWNTs). The mechanisms of different DNA-protein interactions that are responsible for the generation of FPSs of MBs were investigated in detail. The proposed strategy can quickly identify the possible sources of FPSs caused by mechanisms other than hybridization in detecting real samples, which would be very helpful in choosing a proper way to modify the structure of the MBs or using a specific inhibitor. The established method was successfully applied to verify the FPSs in the measurement of a plant tissue sample. (C) 2011 Elsevier B.V. All rights reserved.
KeywordMolecular Beacon False-positive Signal Single Walled Carbon Nanotube Nuclease Degradation
DOI10.1016/j.bios.2011.02.009
Indexed BySCI
Language英语
WOS IDWOS:000289863900033
PublisherELSEVIER ADVANCED TECHNOLOGY
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/73420
Collection中国科学院化学研究所
Corresponding AuthorZhao, Meiping
AffiliationPeking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Su, Xin,Zhang, Chen,Zhao, Meiping. Discrimination of the false-positive signals of molecular beacons by combination of heat inactivation and using single walled carbon nanotubes[J]. BIOSENSORS & BIOELECTRONICS,2011,26(8):3596-3601.
APA Su, Xin,Zhang, Chen,&Zhao, Meiping.(2011).Discrimination of the false-positive signals of molecular beacons by combination of heat inactivation and using single walled carbon nanotubes.BIOSENSORS & BIOELECTRONICS,26(8),3596-3601.
MLA Su, Xin,et al."Discrimination of the false-positive signals of molecular beacons by combination of heat inactivation and using single walled carbon nanotubes".BIOSENSORS & BIOELECTRONICS 26.8(2011):3596-3601.
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