ICCAS OpenIR
Direct energy transfer from conjugated polymer to DNA intercalated dye: Label-free fluorescent DNA detection
Wang, Bing; Yang, Qiong; Liu, Libing; Wang, Shu
2011-06-15
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume85Issue:1Pages:8-11
AbstractThe development of methods for DNA detection is of importance in disease diagnosis, gene-targeted drug discovery and molecular biology field. In this paper, we synthesize a new cationic water-soluble CP containing fluorene moiety and flexible ethylenic moiety in the backbone (PRO for label-free DNA detection. The conformational freedom of PFV provides stronger interactions with double-stranded DNA (dsDNA) and optimizes the orientation of transition moments between PFV and ethidium bromide (EB) intercalated in dsDNA. The efficient FRET from PFV (donor) to EB (acceptor) intercalated in dsDNA is observed and the emission of EB is amplified by the good light-harvesting ability of conjugated polymers. The interactions between PFV and DNA can also be probed by measuring the FRET ratio between PFV and EB intercalated in DNA. In comparison to other DNA detection assays based on FRET and conjugated polymers, synthesis of dye-labeled DNA probe is avoided in our method, which significantly reduces the cost and the synthetic complexity. The PFV/dsDNA/EB system provides promising applications on DNA detection with a simply, fast and label-free manner. (C) 2010 Elsevier B.V. All rights reserved.
KeywordDna Conjugated Polymers Fret Fluorescent Sensors
DOI10.1016/j.colsurfb.2010.10.026
Indexed BySCI
Language英语
WOS IDWOS:000290196100003
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/73338
Collection中国科学院化学研究所
Corresponding AuthorYang, Qiong
AffiliationChinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Bing,Yang, Qiong,Liu, Libing,et al. Direct energy transfer from conjugated polymer to DNA intercalated dye: Label-free fluorescent DNA detection[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2011,85(1):8-11.
APA Wang, Bing,Yang, Qiong,Liu, Libing,&Wang, Shu.(2011).Direct energy transfer from conjugated polymer to DNA intercalated dye: Label-free fluorescent DNA detection.COLLOIDS AND SURFACES B-BIOINTERFACES,85(1),8-11.
MLA Wang, Bing,et al."Direct energy transfer from conjugated polymer to DNA intercalated dye: Label-free fluorescent DNA detection".COLLOIDS AND SURFACES B-BIOINTERFACES 85.1(2011):8-11.
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