ICCAS OpenIR
Dynamic behavior of small heat shock protein inhibition on amyloid fibrillization of a small peptide (SSTSAA) from RNase A
Xi, Dong1,2; Dong, Xiao1; Deng, Wei1; Lai, Luhua1,2
2011-12-09
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume416Issue:1-2Pages:130-134
AbstractSmall heat shock proteins, a class of molecular chaperones, are reported to inhibit amyloid fibril formation in vitro, while the mechanism of inhibition remains unknown. In the present study, we investigated the mechanism by which Mj HSP16.5 inhibits amyloid fibril formation of a small peptide (SSTSAA) from RNase A. A model peptide (dansyl-SSTSAA-W) was designed by introducing a pair of fluorescence resonance energy transfer (FRET) probes into the peptide, allowing for the monitoring of fibril formation by this experimental model. Mj HSP16.5 completely inhibited fibril formation of the model peptide at a molar ratio of 1:120. The dynamic process of fibril formation, revealed by FRET, circular dichroism, and electron microscopy, showed a lag phase of about 2 h followed by a fast growth period. The effect of Mj HSP16.5 on amyloid fibril formation was investigated by adding it into the incubation solution during different growth phases. Adding Mj HSP16.5 to the incubating peptide before or during the lag phase completely inhibited fibril formation. However, introducing Mj HSP16.5 after the lag phase only slowed down the fibril formation process by adhering to the already formed fibrils. These findings provide insight into the inhibitory roles of small heat shock proteins on amyloid fibril formation at the molecular level. (C) 2011 Elsevier Inc. All rights reserved.
KeywordAmyloid Fibril Formation Inhibition Mj Hsp16.5 Small Heat Shock Protein Fret Electron Microscopy
DOI10.1016/j.bbrc.2011.11.010
Indexed BySCI
Language英语
WOS IDWOS:000298222100022
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/73226
Collection中国科学院化学研究所
Corresponding AuthorLai, Luhua
Affiliation1.Peking Univ, BNLMS, State Key Lab Struct Chem Unstable & Stable Speci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Peking Univ, Ctr Theoret Biol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Xi, Dong,Dong, Xiao,Deng, Wei,et al. Dynamic behavior of small heat shock protein inhibition on amyloid fibrillization of a small peptide (SSTSAA) from RNase A[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,416(1-2):130-134.
APA Xi, Dong,Dong, Xiao,Deng, Wei,&Lai, Luhua.(2011).Dynamic behavior of small heat shock protein inhibition on amyloid fibrillization of a small peptide (SSTSAA) from RNase A.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,416(1-2),130-134.
MLA Xi, Dong,et al."Dynamic behavior of small heat shock protein inhibition on amyloid fibrillization of a small peptide (SSTSAA) from RNase A".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 416.1-2(2011):130-134.
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