ICCAS OpenIR
Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery
Lee, Yuhan1,2; Lee, Soo Hyeon1,2; Kim, Jee Seon1,2; Maruyama, Atsushi3,4; Chen, Xuesi5; Park, Tae Gwan1,2
2011-10-10
Source PublicationJOURNAL OF CONTROLLED RELEASE
ISSN0168-3659
Volume155Issue:1Pages:3-10
AbstractDevelopment of nano-sized gene delivery vehicles for small interfering RNA (siRNA) delivery is of great importance for their clinical applications such as cancer therapy. Herein, we demonstrate the controlled synthesis of polyethyleneimine (PEI)-coated gold nanoparticles (AuNPs) using catechol-conjugated PEI (PEI-C) for siRNA delivery. Since the conjugated catechol groups are reductive and moderately hydrophobic. PEI-C formed spherical multi-cored micelles in aqueous solution and served as reductive templates for the growth and synthesis of spherical AuNPs with tunable sizes and surface charges. PEI-C was stably anchored on the surface of growing crystal gold seeds with crosslinking, resulting in robust cationic AuNPs. The fabricated PEI-coated AuNPs formed stable complexes with siRNA, and the complexes showed an excellent gene silencing effect in cancer cells. Size and surface charge values of the synthesized AuNPs had a great influence on intracellular uptake and unpacking of siRNA, and the resultant gene silencing efficiency. The PEI-coated AuNPs exhibited an extremely low cytotoxicity due to the reduced density of primary amine groups and the absence of uncomplexed PEI fraction in aqueous solution. (C) 2010 Elsevier B.V. All rights reserved.
KeywordGold Nanoparticles Polyethylenimine Catechol Sirna Gene Delivery
DOI10.1016/j.jconrel.2010.09.009
Indexed BySCI
Language英语
WOS IDWOS:000295998200003
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:73[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/73032
Collection中国科学院化学研究所
Corresponding AuthorPark, Tae Gwan
Affiliation1.Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea
2.Korea Adv Inst Sci & Technol, Grad Sch Nanosci & Technol, Taejon 305701, South Korea
3.Kyushu Univ, Inst Mat Chem & Engn, Higashi Ku, Fukuoka 8128581, Japan
4.JST, CREST, Higashi Ku, Fukuoka 8128581, Japan
5.Chinese Acad Sci, Changchun Inst Appl Chem, Grad Sch, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
Recommended Citation
GB/T 7714
Lee, Yuhan,Lee, Soo Hyeon,Kim, Jee Seon,et al. Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery[J]. JOURNAL OF CONTROLLED RELEASE,2011,155(1):3-10.
APA Lee, Yuhan,Lee, Soo Hyeon,Kim, Jee Seon,Maruyama, Atsushi,Chen, Xuesi,&Park, Tae Gwan.(2011).Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery.JOURNAL OF CONTROLLED RELEASE,155(1),3-10.
MLA Lee, Yuhan,et al."Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery".JOURNAL OF CONTROLLED RELEASE 155.1(2011):3-10.
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