ICCAS OpenIR
Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides
Huang, Yanyan1; Zhao, Rui1; Fu, Yabin2; Zhang, Qundan1; Xiong, Shaoxiang1; Li, Li2; Zhou, Rouli2; Liu, Guoquan1; Chen, Yi1
2011-05-16
Source PublicationCHEMBIOCHEM
ISSN1439-4227
Volume12Issue:8Pages:1209-1215
AbstractSpecific detection and in vivo tracing of cancer biomarkers are important for cancer analysis. In this work, a simple and effective strategy for developing peptide probes was established. Peptides were rationally designed by using an antisense peptide approach directed towards an extracellular fragment (EL2) of a novel tumor-related protein LAPTM4B. Positional-scanning and stepwise affinity screening was employed to obtain an optimal peptide AP2H (IHGHHIISVG). The dissociation constant between the two small peptides, AP2H and the target EL2, was 5.51 mu m under physiological conditions. Fluorescence imaging assays indicated that AP2H can recognize live hepatoma cells by targeting the LAPTM4B protein on the cell surface with high specificity, low cytotoxicity and desirable cell penetrability. Compared to negative control cells, AP2H could differentiate cells with different expression levels of LAPTM4B. The screened peptide probe for molecular signatures of cancer cells, based on targeting the LAPTM4B protein, has potential applications in cancer diagnosis and targetable drug delivery.
KeywordAffinity Chromatography Cancer Cell Imaging Molecular Recognition Peptides
DOI10.1002/cbic.201100031
Indexed BySCI
Language英语
WOS IDWOS:000290862400011
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72814
Collection中国科学院化学研究所
Corresponding AuthorZhao, Rui
Affiliation1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, CAS Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China
2.Peking Univ, Dept Cell Biol, Sch Basic Med Sci, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Huang, Yanyan,Zhao, Rui,Fu, Yabin,et al. Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides[J]. CHEMBIOCHEM,2011,12(8):1209-1215.
APA Huang, Yanyan.,Zhao, Rui.,Fu, Yabin.,Zhang, Qundan.,Xiong, Shaoxiang.,...&Chen, Yi.(2011).Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides.CHEMBIOCHEM,12(8),1209-1215.
MLA Huang, Yanyan,et al."Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides".CHEMBIOCHEM 12.8(2011):1209-1215.
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