ICCAS OpenIR
Ratiometric Fluorescent Sensor Based on Inhibition of Resonance for Detection of Cadmium in Aqueous Solution and Living Cells
Xue, Lin1; Li, Guoping1,3; Liu, Qing1,3; Wang, Huanhuan1,3; Liu, Chun2; Ding, Xunlei1; He, Shenggui1; Jiang, Hua1
2011-04-18
Source PublicationINORGANIC CHEMISTRY
ISSN0020-1669
Volume50Issue:8Pages:3680-3690
AbstractAlthough cadmium has been recognized as a highly toxic heavy metal and poses many detrimental effects on human health, the Cd2+-uptake and nosogenesis mechanisms are still insufficiently understood, mainly because of the lack of facile analytical methods for monitoring changes in the environmental and intracellular Cd2+ concentrations with high spatial and temporal reliability. To this end, we present the design, synthesis, and photophysical properties of a cadmium sensor, DQCd1 based on the fluorophore 4-isobutoxy-6-(dimethylamino)-8-methoxyquinaldine (model compound 1). Preliminary investigations indicate that 1 could be protonated under neutral media and yield a resonance process over the quinoline fluorophore. Upon excitation at 405 nm, 1 shows a strong fluorescence emission at 554 nm with a quantum yield of 0.17. Similarly, DQCd1 bears properties comparable to its precursor. It exhibits fluorescence emission at 558 nm (Phi(f) = 0.15) originating from the monocationic species under physiological conditions. Coordination with Cd2+ causes quenching of the emission at 558 nm and simultaneously yields a significant hypsochromic shift of the emission maximum to 495 nm (Phi(f) = 0.11) due to inhibition of the resonance process. Thus, a single-excitation, dual-emission ratiometric measurement with a large blue shift in emission (Delta lambda = 63 nm) and remarkable changes in the ratio (F-495 nm/F-558 nm) of the emission intensity (R/R-0 up to 15-fold) is established. Moreover, the sensor DQCd1 exhibits very high sensitivity for Cd2+ (K-d = 41 pM) and excellent selectivity response for Cd2+ over other heavy- and transition-metal ions and Na+, K+, Mg2+ and Ca2+ at the millimolar level. Therefore, DQCd1 can act as a ratiometric fluorescent sensor for Cd2+ through inhibition of the resonance process. Confocal microscopy and cytotoxicity experiments indicate that DQCd1 is cell-permeable and noncytotoxic under our experimental conditions. It can indeed visualize the changes of intracellular Cd2+ in living cells using dual-emission ratiometry.
DOI10.1021/ic200032e
Indexed BySCI
Language英语
WOS IDWOS:000290457700060
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:101[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72718
Collection中国科学院化学研究所
Corresponding AuthorJiang, Hua
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Xue, Lin,Li, Guoping,Liu, Qing,et al. Ratiometric Fluorescent Sensor Based on Inhibition of Resonance for Detection of Cadmium in Aqueous Solution and Living Cells[J]. INORGANIC CHEMISTRY,2011,50(8):3680-3690.
APA Xue, Lin.,Li, Guoping.,Liu, Qing.,Wang, Huanhuan.,Liu, Chun.,...&Jiang, Hua.(2011).Ratiometric Fluorescent Sensor Based on Inhibition of Resonance for Detection of Cadmium in Aqueous Solution and Living Cells.INORGANIC CHEMISTRY,50(8),3680-3690.
MLA Xue, Lin,et al."Ratiometric Fluorescent Sensor Based on Inhibition of Resonance for Detection of Cadmium in Aqueous Solution and Living Cells".INORGANIC CHEMISTRY 50.8(2011):3680-3690.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xue, Lin]'s Articles
[Li, Guoping]'s Articles
[Liu, Qing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xue, Lin]'s Articles
[Li, Guoping]'s Articles
[Liu, Qing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xue, Lin]'s Articles
[Li, Guoping]'s Articles
[Liu, Qing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.