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Enhancing the Catalytic Activity of Peroxidase by Adsorption onto Fe3O4 Magnetic Nanoparticle/Multiwalled Carbon Nanotube Composite Surfaces
Gao Yun-Yan1; Li Hai-Xia1; Ou Zhi-Ze1; Hao Ping1; Li Yi2; Yang Guo-Qiang3
2011-10-01
Source PublicationACTA PHYSICO-CHIMICA SINICA
ISSN1000-6818
Volume27Issue:10Pages:2469-2477
AbstractFe3O4 magnetic nanoparticles were prepared by co-precipitation and supported on the surface of aminopyridine-grafted multiwalled carbon nanotubes (MWCNT-AP), affording a superparamagnetic Fe3O4/MWCNT-AP composite with good dispersity. The Fe3O4/MWCNT-AP composites were characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and hysteresis curve measurements. Transmission electron microscopy (TEM) experimental results suggest that the Fe3O4 nanoparticles are mainly adsorbed on the tips of the MWCNT-AP. UV-Vis absorption and fluorescence results demonstrate that horseradish peroxidase (HRP) can be physically adsorbed onto the surfaces of the Fe3O4/MWCNT-AP composite. Under acidic conditions (pH 4.0), the maximum reaction rate (v(max)) value of the HRP increases 3-fold after interaction with Fe3O4/MWCNT-AP.
KeywordCarbon Nanotube Magnetic Nanoparticle Peroxidase Nanocomposite Superparamagnetic Property
DOI10.3866/PKU.WHXB20110939
Indexed BySCI
Language英语
WOS IDWOS:000295380300032
PublisherPEKING UNIV PRESS
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72714
Collection中国科学院化学研究所
Corresponding AuthorGao Yun-Yan
Affiliation1.Northwestern Polytech Univ, Sch Sci, Minist Educ, Key Lab Space Appl Phys & Chem, Xian 710072, Peoples R China
2.Chinese Acad Sci, Key Lab Photochem Convers & Optoelect Mat, Tech Inst Phys & Chem, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Inst Chem, Key Lab Photochem, Beijing, Peoples R China
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GB/T 7714
Gao Yun-Yan,Li Hai-Xia,Ou Zhi-Ze,et al. Enhancing the Catalytic Activity of Peroxidase by Adsorption onto Fe3O4 Magnetic Nanoparticle/Multiwalled Carbon Nanotube Composite Surfaces[J]. ACTA PHYSICO-CHIMICA SINICA,2011,27(10):2469-2477.
APA Gao Yun-Yan,Li Hai-Xia,Ou Zhi-Ze,Hao Ping,Li Yi,&Yang Guo-Qiang.(2011).Enhancing the Catalytic Activity of Peroxidase by Adsorption onto Fe3O4 Magnetic Nanoparticle/Multiwalled Carbon Nanotube Composite Surfaces.ACTA PHYSICO-CHIMICA SINICA,27(10),2469-2477.
MLA Gao Yun-Yan,et al."Enhancing the Catalytic Activity of Peroxidase by Adsorption onto Fe3O4 Magnetic Nanoparticle/Multiwalled Carbon Nanotube Composite Surfaces".ACTA PHYSICO-CHIMICA SINICA 27.10(2011):2469-2477.
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