ICCAS OpenIR
Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE
Xia Zhi-Ning1,2; Li Li-Xian1,2; Chen Hua2; Xiong Cai-Qiao3; Yang Feng-Qing2
2011-04-10
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN0251-0790
Volume32Issue:4Pages:851-856
AbstractBased on the theory of plug-plug kinetic capillary electrophoresis(ppKCE), the rabbit red blood cell membrane and human red blood cell membrane were used to study the kinetic parameters of the interaction between the cell membrane and drug. The rate constants of complex formation k(on), the dissociation k(off) and the binding constant K(b) between cell membrane and drugs were directly determined simultaneously. The stability of peak height and migration time was studied. The influence of pH and temperature on the kinetic parameters of the interaction between the cell membrane and drugs were examined and analyzed. The results indicate that at physiological pH value, the cell membrane showed biggest biology activeness, the k(on) and K(b) between cell membrane and drugs reached maximum value. The values of k(off), k(on) and K(b) between cell membrane and CIT, Epinastine, Gatifloxacin and Roxatidine were gained. The accuracy of ppKCE was confirmed by the time ratio method and UV-Vis absorption spectra. It could provide a new approach for the evaluation of drug efficiency, toxicity and pharmacokinetics in vivo.
KeywordPlug-plug Kinetic Capillary Electrophoresis (Ppkce) Cell Membrane Kinetic Parameter Interaction
Indexed BySCI
Language英语
WOS IDWOS:000290076100011
PublisherHIGHER EDUCATION PRESS
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72710
Collection中国科学院化学研究所
Corresponding AuthorXia Zhi-Ning
Affiliation1.Chongqing Univ, Coll Bioengn, Chongqing 400030, Peoples R China
2.Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400030, Peoples R China
3.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xia Zhi-Ning,Li Li-Xian,Chen Hua,et al. Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2011,32(4):851-856.
APA Xia Zhi-Ning,Li Li-Xian,Chen Hua,Xiong Cai-Qiao,&Yang Feng-Qing.(2011).Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,32(4),851-856.
MLA Xia Zhi-Ning,et al."Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 32.4(2011):851-856.
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