ICCAS OpenIR
In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection
Bai, Yu; Du, Fuyou; Yang, Youyou; Bai, Yu; Liu, Huwei
2011-10-01
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume34Issue:20Pages:2893-2900
AbstractQuantum dots (QDs), with their superior size-dependent fluorescence properties, have been employed as non-covalent fluorescent labels for the determination of tomato systemin (TomSys) by capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection. The optimum conditions of in-capillary labeling and CE separation were investigated in detail, and complete separation of QDs-labeled TomSys from free QDs labels was achieved. Satisfactory results were obtained in terms of linearity (R(2) = 0.998), sensitivity (limit of detection, 66 fmol) and repeatability (run-to-run RSDs of migration time and peak area, 0.9 and 4.6%, respectively; day-to-day RSDs of migration time and peak area, 3.1 and 11.9%, respectively). The established CE-LIF method was later applied in the detection of TomSys spiked in the sample of tomato leaves, which showed the applicability of the proposed method in the analysis of the target plant peptide hormone in the complex matrix.
KeywordCe Non-covalent Labeling Plant Peptide Hormone Quantum Dots Systemin
DOI10.1002/jssc.201100551
Indexed BySCI
Language英语
WOS IDWOS:000296905400015
PublisherWILEY-BLACKWELL
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72536
Collection中国科学院化学研究所
Corresponding AuthorLiu, Huwei
AffiliationPeking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Key Lab Bioorgan Chem & Mol Engn,Inst Analyt Chem, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Bai, Yu,Du, Fuyou,Yang, Youyou,et al. In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(20):2893-2900.
APA Bai, Yu,Du, Fuyou,Yang, Youyou,Bai, Yu,&Liu, Huwei.(2011).In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection.JOURNAL OF SEPARATION SCIENCE,34(20),2893-2900.
MLA Bai, Yu,et al."In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection".JOURNAL OF SEPARATION SCIENCE 34.20(2011):2893-2900.
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