ICCAS OpenIR
Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation
Cui, Yi-Ran1; Hong, Chao2; Zhou, Ying-Lin1; Li, Yue3; Gao, Xiao-Ming2,3; Zhang, Xin-Xiang1
2011-09-15
Source PublicationTALANTA
ISSN0039-9140
Volume85Issue:3Pages:1246-1252
AbstractOrientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles were synthesized for cell separation. The Fe3O4@Au magnetic nanoparticles were synthesized by reducing HAuCl4 on the surfaces of Fe3O4 nanoparticles, which were further characterized in detail by TEM, XRD and UV-vis spectra. Anti-CD3 monoclonal antibody was orientedly bioconjugated to the surface of Fe3O4@Au nanoparticles through affinity binding between the Fc portion of the antibody and protein A that covalently immobilized on the nanoparticles. The oriented immobilization method was performed to compare its efficiency for cell separation with the non-oriented one, in which the antibody was directly immobilized onto the carboxylated nanoparticle surface. Results showed that the orientedly bioconjugated Fe3O4@Au MNPs successfully pulled down CD3(+) T cells from the whole splenocytes with high efficiency of up to 98.4%, showing a more effective cell-capture nanostructure than that obtained by non-oriented strategy. This developed strategy for the synthesis and oriented bioconjugation of Fe3O4@Au MNPs provides an efficient tool for cell separation, and may be further applied to various fields of bioanalytical chemistry for diagnosis, affinity extraction and biosensor. (C) 2011 Elsevier B.V. All rights reserved.
KeywordFe3o4/au Magnetic Nanoparticles (Fe3o4@au Mnps) Oriented Immobilization Antibody Protein a Cell Separation
DOI10.1016/j.talanta.2011.05.010
Indexed BySCI
Language英语
WOS IDWOS:000294092800005
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:66[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72434
Collection中国科学院化学研究所
Corresponding AuthorZhang, Xin-Xiang
Affiliation1.Peking Univ, BNLMS, Key Lab Biochem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem, Beijing 100871, Peoples R China
2.Soochow Univ, Inst Biol & Med Sci, Suzhou 215123, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Cui, Yi-Ran,Hong, Chao,Zhou, Ying-Lin,et al. Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation[J]. TALANTA,2011,85(3):1246-1252.
APA Cui, Yi-Ran,Hong, Chao,Zhou, Ying-Lin,Li, Yue,Gao, Xiao-Ming,&Zhang, Xin-Xiang.(2011).Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation.TALANTA,85(3),1246-1252.
MLA Cui, Yi-Ran,et al."Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation".TALANTA 85.3(2011):1246-1252.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Cui, Yi-Ran]'s Articles
[Hong, Chao]'s Articles
[Zhou, Ying-Lin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Cui, Yi-Ran]'s Articles
[Hong, Chao]'s Articles
[Zhou, Ying-Lin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Cui, Yi-Ran]'s Articles
[Hong, Chao]'s Articles
[Zhou, Ying-Lin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.