ICCAS OpenIR
Reduction-Degradable Linear Cationic Polymers as Gene Carriers Prepared by Cu(I)-Catalyzed Azide-Alkyne Cycloaddition
Wang, Yang1; Zhang, Rui1; Xu, Ning1; Du, Fu-Sheng1; Wang, Ying-Li2; Tan, Ying-Xia2; Ji, Shou-Ping2; Liang, De-Hai1; Li, Zi-Chen1
2011
Source PublicationBIOMACROMOLECULES
ISSN1525-7797
Volume12Issue:1Pages:66-74
AbstractLinear reduction-degradable cationic polymers with different secondary amine densities (S2 and S3) and their nonreducible counterparts (C2 and C3) were synthesized by Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) step-growth polymerization of the dialkyne-oligoamine monomers and the diazide monomers. These polymers were studied with a goal of developing a set of new gene carriers. The buffering capacity and DNA binding ability of these polymers were evaluated by acid base titration, gel retardation, and ethidium bromide (EB) exclusion assay. The polymers with lower amine density exhibit a weaker DNA-binding ability but a stronger buffering capacity in the range of pH 5.1 and 7.4. Particle size and zeta-potential measurements demonstrate that the polymers with higher amine density condense pDNA to form polyplexes with smaller sizes, while the disulfide bond in the backbone shows a negative effect on the condensing capability of the polymers, resulting in the formation of polyplexes with large size and nearly neutral surface. The reduction-sensitive polyplexes formed by polymer S2 of S3 can be disrupted by dithiothreitol (DTT) to release free DNA, which has been proven by the combination of gel retardation, EB exclusion assay, particles sizing, and zeta potential measurements. Cell viability measurements by MTT assay demonstrate that the reduction-degradable polymers (S2 and S3) have little cytotoxicity while the nonreducible polymers (C2 and C3) show obvious cytotoxicity, in particular, at high N/P ratios. In vitro transfection efficiencies of these polymers were evaluated using EGFP and luciferase plasmids as the reporter genes. Polymers S3 and S2 show much higher efficiencies than the nonreducible polymers C3 and C2 in the absence of 10% serum; unexpectedly, the lowest transfection efficiency has been observed for polymer S3 in the presence of serum.
DOI10.1021/bm101005j
Indexed BySCI ; IC
Language英语
WOS IDWOS:000285956700011
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72430
Collection中国科学院化学研究所
Corresponding AuthorDu, Fu-Sheng
Affiliation1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Polymer Chem & Phys, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Beijing Inst Transfus Med, Dept Mol Biol, Beijing 100850, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yang,Zhang, Rui,Xu, Ning,et al. Reduction-Degradable Linear Cationic Polymers as Gene Carriers Prepared by Cu(I)-Catalyzed Azide-Alkyne Cycloaddition[J]. BIOMACROMOLECULES,2011,12(1):66-74.
APA Wang, Yang.,Zhang, Rui.,Xu, Ning.,Du, Fu-Sheng.,Wang, Ying-Li.,...&Li, Zi-Chen.(2011).Reduction-Degradable Linear Cationic Polymers as Gene Carriers Prepared by Cu(I)-Catalyzed Azide-Alkyne Cycloaddition.BIOMACROMOLECULES,12(1),66-74.
MLA Wang, Yang,et al."Reduction-Degradable Linear Cationic Polymers as Gene Carriers Prepared by Cu(I)-Catalyzed Azide-Alkyne Cycloaddition".BIOMACROMOLECULES 12.1(2011):66-74.
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