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A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance
Zhang, Meng1,2; Lin, Shixian1; Song, Xinwen2; Liu, Jun1; Fu, Ye3; Ge, Xi2; Fu, Xinmiao2,4; Chang, Zengyi2,4; Chen, Peng R.1,4,5
2011-10-01
Source PublicationNATURE CHEMICAL BIOLOGY
ISSN1552-4450
Volume7Issue:10Pages:671-677
AbstractAcid chaperones are essential factors in preserving the protein homeostasis for enteric pathogens to survive in the extremely acidic mammalian stomach (pH 1-3). The client proteins of these chaperones remain largely unknown, primarily because of the exceeding difficulty of determining protein-protein interactions under low-pH conditions. We developed a genetically encoded, highly efficient protein photocrosslinking probe, which enabled us to profile the in vivo substrates of a major acid-protection chaperone, HdeA, in Escherichia coli periplasm. Among the identified HdeA client proteins, the periplasmic chaperones DegP and SurA were initially found to be protected by HdeA at a low pH, but they subsequently facilitated the HdeA-mediated acid recovery of other client proteins. This unique, ATP-independent chaperone cooperation in the ATP-deprived E. coli periplasm may support the acid resistance of enteric bacteria. The crosslinker would be valuable in unveiling the physiological interaction partners of any given protein and thus their functions under normal and stress conditions.
DOI10.1038/NCHEMBIO.644
Indexed BySCI
Language英语
WOS IDWOS:000295027100010
PublisherNATURE PUBLISHING GROUP
Citation statistics
Cited Times:130[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72396
Collection中国科学院化学研究所
Corresponding AuthorChen, Peng R.
Affiliation1.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Peking Univ, State Key Lab Prot & Plant Gene Res, Sch Life Sci, Beijing 100871, Peoples R China
3.Univ Chicago, Dept Chem, Chicago, IL 60637 USA
4.Peking Univ, Ctr Prot Sci, Beijing 100871, Peoples R China
5.Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Meng,Lin, Shixian,Song, Xinwen,et al. A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance[J]. NATURE CHEMICAL BIOLOGY,2011,7(10):671-677.
APA Zhang, Meng.,Lin, Shixian.,Song, Xinwen.,Liu, Jun.,Fu, Ye.,...&Chen, Peng R..(2011).A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance.NATURE CHEMICAL BIOLOGY,7(10),671-677.
MLA Zhang, Meng,et al."A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance".NATURE CHEMICAL BIOLOGY 7.10(2011):671-677.
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