ICCAS OpenIR
Surface-biofunctionalized multicore/shell CdTe@SiO2 composite particles for immunofluorescence assay
Jing, Lihong1; Li, Yilin1; Ding, Ke1; Qiao, Ruirui1; Rogach, Andrey L.2,3; Gao, Mingyuan1
2011-12-16
Source PublicationNANOTECHNOLOGY
ISSN0957-4484
Volume22Issue:50
AbstractStrongly fluorescent multicore/shell structured CdTe@SiO2 composite particles of similar to 50 nm were synthesized via the reverse microemulsion method by using CdTe quantum dots co-stabilized by thioglycolic acid and thioglycerol. The optical stability of the CdTe@SiO2 composite particles in a wide pH range, under prolonged UV irradiation in pure water, or in different types of physiological buffers was systematically investigated. Towards immunofluorescence assay, both poly(ethylene glycol) (PEG) and carboxyl residues were simultaneously grafted on the surface of the silanol-terminated CdTe@SiO2 composite particles upon further reactions with silane reagents bearing a PEG segment and carboxyl group, respectively, in order to suppress the nonspecific interactions of the silica particles with proteins and meanwhile introduce reactive moieties to the fluorescent particles. Agarose gel electrophoresis, dynamic light scattering and conventional optical spectroscopy were combined to investigate the effectiveness of the surface modifications. Via the surface carboxyl residue, various antibodies were covalently conjugated to the fluorescent particles and the resultant fluorescent probes were used in detecting cancer cells through both direct fluorescent antibody and indirect fluorescent antibody assays, respectively.
DOI10.1088/0957-4484/22/50/505104
Indexed BySCI
Language英语
WOS IDWOS:000298154200004
PublisherIOP PUBLISHING LTD
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72312
Collection中国科学院化学研究所
Corresponding AuthorGao, Mingyuan
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
2.City Univ Hong Kong, Dept Phys & Mat Sci, Kowloon, Hong Kong, Peoples R China
3.City Univ Hong Kong, Ctr Funct Photon, Kowloon, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Jing, Lihong,Li, Yilin,Ding, Ke,et al. Surface-biofunctionalized multicore/shell CdTe@SiO2 composite particles for immunofluorescence assay[J]. NANOTECHNOLOGY,2011,22(50).
APA Jing, Lihong,Li, Yilin,Ding, Ke,Qiao, Ruirui,Rogach, Andrey L.,&Gao, Mingyuan.(2011).Surface-biofunctionalized multicore/shell CdTe@SiO2 composite particles for immunofluorescence assay.NANOTECHNOLOGY,22(50).
MLA Jing, Lihong,et al."Surface-biofunctionalized multicore/shell CdTe@SiO2 composite particles for immunofluorescence assay".NANOTECHNOLOGY 22.50(2011).
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