ICCAS OpenIR
Method for detection of Hg2+ based on the specific thymine-Hg2+-thymine interaction in the DNA hybridization on the surface of quartz crystal microbalance
Sheng, Zhonghan2; Han, Jianhua2; Zhang, Jianping2; Zhao, Hong1; Jiang, Long2
2011-10-15
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume87Issue:2Pages:289-292
AbstractA simple and sensitive method was developed for the detection of mercury ions with quartz crystal microbalance (QCM), based on the specific thymine-Hg2(+)-thymine (T-Hg2+-T) interaction and gold nanoparticle-mediated signal amplification. To enhance the sensitivity of detection a sandwich hybridization approach was adopted in this work. The QCM gold surface was modified with the probe SH-oligonucleotides (Oligo-1) and 6-Mercapto-1-hexanol to form an active surface for the hybridization of a longer ss-DNA (Oligo-2), and then Oligo-3 hybridazated with an excess and matching part of Oligo-2. In all oligonucleotides, there existed T bases. In the presence of Hg2+ ions, special T-Hg2+-T reaction greatly enhanced the hybridization of oligonucleotides and detection sensitivity. The gold nanoparticle (Au NPs) amplifier method further increased the sensitivity of detection. A detection sensitivity of 5 nM Hg2+ was obtained in the QCM system, whereas other coexisting metal ions (such as Ni2+, Mg2+, Co2+, Cr3+, Pb2+, Cd2+, Mn2+, Ba2+) had no significant interference. This method reveals a new approach for the manufacture of a kind of simple and low cost sensors for the Hg2+ detection. (C) 2011 Elsevier B.V. All rights reserved.
KeywordQuartz Crystal Microbalance Hg2++++ Ion Gold Nanoparticle Thymine
DOI10.1016/j.colsurfb.2011.05.031
Indexed BySCI
Language英语
WOS IDWOS:000294142900011
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72290
Collection中国科学院化学研究所
Corresponding AuthorZhao, Hong
Affiliation1.Chinese Acad Sci, Grad Univ, Coll Chem & Chem Engn, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Chem, Key Lab Colloid & interface Sci, BNLMS, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Sheng, Zhonghan,Han, Jianhua,Zhang, Jianping,et al. Method for detection of Hg2+ based on the specific thymine-Hg2+-thymine interaction in the DNA hybridization on the surface of quartz crystal microbalance[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2011,87(2):289-292.
APA Sheng, Zhonghan,Han, Jianhua,Zhang, Jianping,Zhao, Hong,&Jiang, Long.(2011).Method for detection of Hg2+ based on the specific thymine-Hg2+-thymine interaction in the DNA hybridization on the surface of quartz crystal microbalance.COLLOIDS AND SURFACES B-BIOINTERFACES,87(2),289-292.
MLA Sheng, Zhonghan,et al."Method for detection of Hg2+ based on the specific thymine-Hg2+-thymine interaction in the DNA hybridization on the surface of quartz crystal microbalance".COLLOIDS AND SURFACES B-BIOINTERFACES 87.2(2011):289-292.
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