ICCAS OpenIR
Determination of formal potential of NADH/NAD(+) redox couple and catalytic oxidation of NADH using poly(phenosafranin)-modified carbon electrodes
Saleh, Farhana S.1; Rahman, Mohammad R.1; Okajima, Takeyoshi1; Mao, Lanqun2; Ohsaka, Takeo1
2011-02-01
Source PublicationBIOELECTROCHEMISTRY
ISSN1567-5394
Volume80Issue:2Pages:121-127
AbstractThe electrochemical regeneration of NADH/NAD(+) redox couple has been studied using poly(phenosafranin) (PPS)-modified carbon electrodes to evaluate the formal potential and catalytic rate constant for the oxidation of NADH. The PPS-modified electrodes were prepared by electropolymerization of phenosafranin onto different carbon substrates (glassy carbon (GC) and basal-plane pyrolytic graphite (BPPG)) in different electrolytic solutions. The formal potential was estimated to be -0.365 +/- 0.002 V vs. SHE at pH 7.0. As for the bare carbon electrodes, the oxidation of NADH at the BPPG electrode was found to be enhanced compared with the GC electrode. For the PPS-modified electrodes, it was found that the electrocatalysis of PPS-modified electrodes for the oxidation of NADH largely depends on the carbon substrate and electrolyte solution employed for their preparation, i.e., the PPS-modified BPPG electrode prepared in 0.2 M NaClO4/acetonitrile solution exhibits an excellent and persistent electrocatalytic property toward NADH oxidation in phosphate buffer solution (pH 7.0) with a diminution of the overpotential of about 740 and 670 mV compared with those at the bare GC electrode and the PPS-modified GC electrode prepared in 0.2 M H2SO4 solution, respectively. A quantitative analysis of the electrocatalytic reaction based on rotating disk voltammetry gave the electrocatalytic reaction rate constants of the order of 10(3)-10(4) M-1 s(-1) depending on the preparation conditions of the PPS-modified electrodes. (C) 2010 Elsevier B.V. All rights reserved.
KeywordNadh/nad(+) Regeneration Formal Potential Poly(Phenosafranin) Carbon Electrodes
DOI10.1016/j.bioelechem.2010.07.001
Indexed BySCI
Language英语
WOS IDWOS:000286851600004
PublisherELSEVIER SCIENCE SA
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72202
Collection中国科学院化学研究所
Corresponding AuthorOhsaka, Takeo
Affiliation1.Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Elect Chem, Midori Ku, Yokohama, Kanagawa 2268502, Japan
2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Saleh, Farhana S.,Rahman, Mohammad R.,Okajima, Takeyoshi,et al. Determination of formal potential of NADH/NAD(+) redox couple and catalytic oxidation of NADH using poly(phenosafranin)-modified carbon electrodes[J]. BIOELECTROCHEMISTRY,2011,80(2):121-127.
APA Saleh, Farhana S.,Rahman, Mohammad R.,Okajima, Takeyoshi,Mao, Lanqun,&Ohsaka, Takeo.(2011).Determination of formal potential of NADH/NAD(+) redox couple and catalytic oxidation of NADH using poly(phenosafranin)-modified carbon electrodes.BIOELECTROCHEMISTRY,80(2),121-127.
MLA Saleh, Farhana S.,et al."Determination of formal potential of NADH/NAD(+) redox couple and catalytic oxidation of NADH using poly(phenosafranin)-modified carbon electrodes".BIOELECTROCHEMISTRY 80.2(2011):121-127.
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