ICCAS OpenIR
Efficient enrichment and identification of phosphopeptides by cerium oxide using on-plate matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis
Sun, Shutao1,2; Ma, Haitao3; Han, Guanghui2; Wu, Ren'an2; Zou, Hanfa2; Liu, Yiwei1
2011-07-15
Source PublicationRAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN0951-4198
Volume25Issue:13Pages:1862-1868
AbstractAn efficient and simple method for enrichment and identification of phosphopeptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) using cerium oxide is presented. After pretreatment of tryptic digests of phosphoproteins with CeO(2), nonphosphopeptides are discarded and phosphopeptides are enriched. By applying the separated CeO(2) on a target plate and analysis using MALDI-TOF MS, peaks of phosphopeptides and their correspondingly series of dephosphorylated peptides are observed in the mass spectra. Thus, the phosphopeptides are very easy to identify with the mass difference, which are all 80 Da between adjacent peaks in the same series, and clear background in the spectra owing to elimination of signal suppression from large amounts of nonphosphopeptides. Furthermore, the phosphopeptides can be dephosphorylated completely after a further NH(4)OH elution. Tryptic digest products from several standard proteins are pretreated using CeO(2) to demonstrate the efficiency of this method. Phosphopeptides from a very small quantity of human serum are enriched and analyzed, and proteins also identified by searching against a database using Mascot on MALDI-TOF/TOF fragments, which indicates that this method may be employed in complex samples for further application. Copyright (C) 2011 John Wiley & Sons, Ltd.
DOI10.1002/rcm.5055
Indexed BySCI
Language英语
WOS IDWOS:000291593900010
PublisherWILEY-BLACKWELL
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/72068
Collection中国科学院化学研究所
Corresponding AuthorSun, Shutao
Affiliation1.Chinese Acad Sci, Inst Microbiol, Lab Struct Microbiol, Core Facil, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
3.Chinese Acad Sci, State Key Lab Mol React Dynam, Ctr Mol Sci, Inst Chem, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Sun, Shutao,Ma, Haitao,Han, Guanghui,et al. Efficient enrichment and identification of phosphopeptides by cerium oxide using on-plate matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2011,25(13):1862-1868.
APA Sun, Shutao,Ma, Haitao,Han, Guanghui,Wu, Ren'an,Zou, Hanfa,&Liu, Yiwei.(2011).Efficient enrichment and identification of phosphopeptides by cerium oxide using on-plate matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,25(13),1862-1868.
MLA Sun, Shutao,et al."Efficient enrichment and identification of phosphopeptides by cerium oxide using on-plate matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 25.13(2011):1862-1868.
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