ICCAS OpenIR
A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles
Xue, Wangxin; Zhang, Guanxin; Zhang, Deqing
2011
Source PublicationANALYST
ISSN0003-2654
Volume136Issue:15Pages:3136-3141
AbstractGold nanoparticles (Au-NPs) with negative charges aggregate in the presence of Arg(6) due to electrostatic interactions resulting in the red-shift of the plasmon absorption. But, after incubation of Arg(6) with trypsin the aggregation of Au-NPs can be prohibited. Accordingly, a newly designed-Au-NPs based colorimetric assay method for trypsin activity is established. Trypsin with a concentration as low as 1.6 ng mL(-1) can be assayed with this new colorimetric assay. This colorimetric label-free assay for trypsin can be performed in aqueous solution and both Au-NPs and Arg(6) are easily accessible. Thus, this assay method is useful for screening inhibitors of trypsin.
DOI10.1039/c1an15224f
Indexed BySCI
Language英语
WOS IDWOS:000292972200014
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:50[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71932
Collection中国科学院化学研究所
Corresponding AuthorZhang, Deqing
AffiliationChinese Acad Sci, Organ Solids Lab, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xue, Wangxin,Zhang, Guanxin,Zhang, Deqing. A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles[J]. ANALYST,2011,136(15):3136-3141.
APA Xue, Wangxin,Zhang, Guanxin,&Zhang, Deqing.(2011).A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles.ANALYST,136(15),3136-3141.
MLA Xue, Wangxin,et al."A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles".ANALYST 136.15(2011):3136-3141.
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