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Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin
Wang Lin1; Li Chun-Yan1; He Pan2; Fu Li1; Zhou Yan-Min1; Chen Xue-Si2
2011-07-10
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN0251-0790
Volume32Issue:7Pages:1622-1628
AbstractBy tripolyphosphate (TPP) as cross-linker, chitosan microspheres (CMs) loaded with adrenomedullin (ADM) were prepared by an emulsion-ionic cross-linking method. Then, porous poly(lactide-co-glycolide) (PLGA)/nano-hydroxyapatite (nHA)/CMs scaffold was developed by thermally induced phase separation. Scanning electron microscope was used to analyze the characterization of the microspheres and the scaffold and the drug release from them was tested in vitro. Also, the hemolytic effect of the scaffold was tested to evaluate the toxicity of the biomaterial. MG63 cell line and HUVEC cell line were cultured for testing the bioactivity of the scaffold loading ADM. After the cells adhered on the scaffold, the cell proliferation and differentiation were analyzed by methyl thiazolyl tetrazolium(MTT) assay, fluorescent staining and alkaline phosphatase(ALP) assay. The results show that the diameter of the microspheres is well-distributed and the PLGA/nHA/CMs scaffold shows a typical microstructure of polymeric foam. The pores of the scaffold ranged from 30 mu m to 220 mu m and they were interconnected. The hemolytic rate of the biomaterial was less than 5%, which was in line with national standard of medical materials. This study confirmed that PLGA/nHA/CMs/ADM and PLGA/nHA/CMs scaffold could accelerate the proliferation of osteoblast-like cells and vascular endothelial cells and also could stimulate the differentiation of osteoblast-like cells.
KeywordChitosan Microsphere Poly(Lactide-co-glycolide) Nano-hydroxyapatite Adrenomedullin
Indexed BySCI
Language英语
WOS IDWOS:000293303800032
PublisherHIGHER EDUCATION PRESS
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71914
Collection中国科学院化学研究所
Corresponding AuthorZhou Yan-Min
Affiliation1.Jilin Univ, Stomatol Hosp, Implant Ctr, Changchun 130021, Peoples R China
2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
Recommended Citation
GB/T 7714
Wang Lin,Li Chun-Yan,He Pan,et al. Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2011,32(7):1622-1628.
APA Wang Lin,Li Chun-Yan,He Pan,Fu Li,Zhou Yan-Min,&Chen Xue-Si.(2011).Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,32(7),1622-1628.
MLA Wang Lin,et al."Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 32.7(2011):1622-1628.
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