Non-specific and specific interactions on functionalized polymer surface studied by FT-SPR
Wei, Jizheng1,2; Yan, Lesan1,2; Hu, Xiuli1; Chen, Xuesi1; Huang, Yubin1; Jing, Xiabin1
AbstractFourier transform surface plasmon resonance (FT-SPR) was utilized to study specific and non-specific interactions between proteins and a biotinylated polymer film by monitoring adsorptions of streptavidin (SAv) and bovine serum albumin (BSA) on the polymer films. The biotinylated polymer, poly(lactide-co-2,2-dihydroxymethyl-propylene carbonate-graft-biotin) [P(LA-co-DHC/biotin)], was prepared by ring-opening copolymerization of lactide and a OH-bearing cyclic carbonate monomer, followed by biotinylation of the OH groups. The copolymer was coated onto the FT-SPR chip and vacuum-dried, hydrated at 70 degrees C, and treated with a blocking agent respectively to achieve different surface status. The FT-SPR results showed that the vacuum-dried film had the most BSA adsorption; hydration treatment led to migration of the biotin moieties from inner film to surface and thus resulted in less BSA adsorption; blocking layer on the polymer surface saturated the active sites for physical and chemical adsorptions on the surface and thus weakened the BSA adsorption. Adsorption of SAv displayed similar polymer-surface-status dependence, i.e., more adsorption on vacuum-dried surface, less adsorption on hydrated surface and the least adsorption on blocked surface. Compared with BSA, SAv showed more enhanced adsorptions on P(LA-co-DHC/biotin) surface because of the specific interaction of biotin moieties in the polymer with SAv molecules, especially on the blocked surface. The above semi-quantified results further indicate that the FT-SPR system is suitable for investigating interactions between polymer surface and bio-molecules. (C) 2010 Elsevier B.V. All rights reserved.
KeywordPolymer Film Fourier Transform Surface Plasmon Resonance Protein Adsorption Biotin-avidin Interaction
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WOS IDWOS:000287268000005
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorJing, Xiabin
Affiliation1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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Wei, Jizheng,Yan, Lesan,Hu, Xiuli,et al. Non-specific and specific interactions on functionalized polymer surface studied by FT-SPR[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2011,83(2):220-228.
APA Wei, Jizheng,Yan, Lesan,Hu, Xiuli,Chen, Xuesi,Huang, Yubin,&Jing, Xiabin.(2011).Non-specific and specific interactions on functionalized polymer surface studied by FT-SPR.COLLOIDS AND SURFACES B-BIOINTERFACES,83(2),220-228.
MLA Wei, Jizheng,et al."Non-specific and specific interactions on functionalized polymer surface studied by FT-SPR".COLLOIDS AND SURFACES B-BIOINTERFACES 83.2(2011):220-228.
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