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Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution
Ge, Jing; Liu, Zhaofang; Zhi, Zeyong; Zhao, Xin Sheng1
2011-06-01
Source PublicationANALYTICAL BIOCHEMISTRY
ISSN0003-2697
Volume413Issue:1Pages:75-77
AbstractIt has been a long-standing challenge in bioassay using aptamers and gold nanoparticles to detect disease-related proteins and other substance directly in complex biological samples such as serum. Here we propose a progressive dilution (PD) method to achieve simultaneous qualitative and quantitative analysis of proteins in blood serum without pretreatment of the sample. Above the detection limit, PD has unlimited dynamic range. We demonstrate the PD strategy through the detection of thrombin in fetal bovine serum using the quenching of fluorescence by gold nanoparticles. (C) 2011 Elsevier Inc. All rights reserved.
DOI10.1016/j.ab.2011.01.032
Indexed BySCI
Language英语
WOS IDWOS:000289607900015
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71692
Collection中国科学院化学研究所
Corresponding AuthorZhao, Xin Sheng
Affiliation1.Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Ge, Jing,Liu, Zhaofang,Zhi, Zeyong,et al. Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution[J]. ANALYTICAL BIOCHEMISTRY,2011,413(1):75-77.
APA Ge, Jing,Liu, Zhaofang,Zhi, Zeyong,&Zhao, Xin Sheng.(2011).Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution.ANALYTICAL BIOCHEMISTRY,413(1),75-77.
MLA Ge, Jing,et al."Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution".ANALYTICAL BIOCHEMISTRY 413.1(2011):75-77.
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