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DNA template-synthesized silver nanoparticles: A new platform for high-performance fluorescent biosensing of biothiols
Jin JianYu1; Ouyang XiangYuan2; Li JiShan2; Jiang JianHui2; Wang Hao1; Wang YongXiang1; Yang RongHua1,2
2011-08-01
Source PublicationSCIENCE CHINA-CHEMISTRY
ISSN1674-7291
Volume54Issue:8Pages:1266-1272
AbstractTo develop the high-performance fluorescent bio-sensors, the metal nanoparticles were employed as nanoquenchers and attracted reasonable attention in the design of fluorescent biosensors. In this work, silver nanoparticles (AgNPs) were obtained via reduction of Ag(+) on FAM-labeled DNA template. For the tight binding between AgNPs and DNA, the template-synthesized AgNPs turned out high quenching efficiency and could be applied as super nanoquenchers to establish the biosensing platform for fluorescent detection. As an example, the template-synthesized DNA-AgNPs conjugates were employed in sensing thiols. By forming S-Ag bonds, thiols interact intensely with AgNPs and replace the FAM-labeled DNA off from the surface of AgNPs, resulting in a fluorescence enhancement. Besides the advantages of lower background and higher signal-to-background ratio (S/B), the conjugates present better stability, making them applicable in complicated biological fluids. To further evidence the feasibility of sensing thiols in real samples, the thiols in human urine were detected. The total amount of free thiols found in human urine was ranging from 229 mu M to 302 mu M with the proposed sensor. To conclude the reliability, low content of Cys was added and the recovery was 98%-103%.
KeywordSilver Nanoparticles Dna Template-synthesized Thiols Fluorescent Sensing
DOI10.1007/s11426-011-4320-0
Indexed BySCI
Language英语
WOS IDWOS:000294062100012
PublisherSCIENCE PRESS
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71566
Collection中国科学院化学研究所
Corresponding AuthorYang RongHua
Affiliation1.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Ctr Biomed Engn, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China
Recommended Citation
GB/T 7714
Jin JianYu,Ouyang XiangYuan,Li JiShan,et al. DNA template-synthesized silver nanoparticles: A new platform for high-performance fluorescent biosensing of biothiols[J]. SCIENCE CHINA-CHEMISTRY,2011,54(8):1266-1272.
APA Jin JianYu.,Ouyang XiangYuan.,Li JiShan.,Jiang JianHui.,Wang Hao.,...&Yang RongHua.(2011).DNA template-synthesized silver nanoparticles: A new platform for high-performance fluorescent biosensing of biothiols.SCIENCE CHINA-CHEMISTRY,54(8),1266-1272.
MLA Jin JianYu,et al."DNA template-synthesized silver nanoparticles: A new platform for high-performance fluorescent biosensing of biothiols".SCIENCE CHINA-CHEMISTRY 54.8(2011):1266-1272.
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