ICCAS OpenIR
Cell affinity for bFGF immobilized heparin-containing poly(lactide-co-glycolide) scaffolds
Shen, Hong; Hu, Xixue; Yang, Fei; Bei, Jianzhong; Wang, Shenguo
2011-05-01
Source PublicationBIOMATERIALS
ISSN0142-9612
Volume32Issue:13Pages:3404-3412
AbstractIn order to effectively and uniformly immobilize basic fibroblast growth factor (bFGF) to thick PLGA scaffold, the heparin-conjugated PLGA (H-PLGA) was synthesized at the first by reaction between heparin and a low molecular weight PLGA. Then heparin-containing PLGA (H-PLGA/PLGA) scaffold was fabricated by blending the H-PLGA with a high molecular weight PLGA. Finally, bFGF was immobilized on the H-PLGA/PLGA scaffold mainly by static electricity action between them. The effect of H-PLGA content on bFGF binding efficiency of the H-PLGA/PLGA scaffolds was investigated. It was found that bFGF binding efficiency increased with increasing H-PLGA content The bound bFGF can release in vitro slowly from the H-PLGA/PLGA scaffolds and last over two weeks. The released bFGF has still preserved its bioactivity. The attachment and growth of mouse 3T3 fibroblasts on the H-PLGA/PLGA scaffolds were better than that on the PLGA scaffold, however bFGF immobilized H-PLGA/PLGA scaffolds showed much better cell affinity. Therefore, the method to use the H-PLGA/PLGA scaffold for immobilizing bFGF is not only effective for slow delivering bFGF with bioactivity, but also can be used for fabricating thick scaffold where bFGF could be combined and uniformly distributed. (C) 2011 Elsevier Ltd. All rights reserved.
KeywordBfgf Immobilization Slow Release Heparin Plga Scaffold
DOI10.1016/j.biomaterials.2011.01.037
Indexed BySCI
Language英语
WOS IDWOS:000288884800007
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:49[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71556
Collection中国科学院化学研究所
Corresponding AuthorWang, Shenguo
AffiliationChinese Acad Sci, Inst Chem, BNLMS, State Key Lab Polymer Phys & Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Shen, Hong,Hu, Xixue,Yang, Fei,et al. Cell affinity for bFGF immobilized heparin-containing poly(lactide-co-glycolide) scaffolds[J]. BIOMATERIALS,2011,32(13):3404-3412.
APA Shen, Hong,Hu, Xixue,Yang, Fei,Bei, Jianzhong,&Wang, Shenguo.(2011).Cell affinity for bFGF immobilized heparin-containing poly(lactide-co-glycolide) scaffolds.BIOMATERIALS,32(13),3404-3412.
MLA Shen, Hong,et al."Cell affinity for bFGF immobilized heparin-containing poly(lactide-co-glycolide) scaffolds".BIOMATERIALS 32.13(2011):3404-3412.
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