ICCAS OpenIR
Colorimetric detection of glucose and an assay for acetylcholinesterase with amine-terminated polydiacetylene vesicles
Xue WangXin; Zhang DeQing; Zhang GuanXin; Zhu DaoBen
2011-06-01
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume56Issue:18Pages:1877-1883
AbstractThe colorimetric response of amine-terminated polydiacetylene (PDA) vesicles was initially demonstrated by varying the pH of the solution. Convenient colorimetric methods to detect glucose and acetylcholinesterase (AChE) activity were successfully established using amine-terminated PDA vesicles by taking advantage of the following features: (1) the amine-terminated PDA vesicles undergo a colorimetric transition as the pH of the solution changes; (2) glucose can be oxidized to gluconic acid in the presence of glucose oxidase; and (3) AChE catalyzes the hydrolysis of acetylcholine to acetic acid. The visual detection of glucose levels and AChE activity showed good selectivity and acceptable sensitivity. The detection limit of glucose was similar to 2.5 mu mol/L and the level of AChE activity was assayed as low as 10.0 mU/mL. Moreover, the amine-terminated PDA vesicles can be used for screening the activity of inhibitors against AChE.
KeywordPolydiacetylene Vesicles Glucose Glucose Oxidase Acetylcholinesterase
DOI10.1007/s11434-011-4505-0
Indexed BySCI
Language英语
WOS IDWOS:000291259500002
PublisherSCIENCE PRESS
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71538
Collection中国科学院化学研究所
Corresponding AuthorZhang DeQing
AffiliationChinese Acad Sci, Inst Chem, Organ Solids Lab, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xue WangXin,Zhang DeQing,Zhang GuanXin,et al. Colorimetric detection of glucose and an assay for acetylcholinesterase with amine-terminated polydiacetylene vesicles[J]. CHINESE SCIENCE BULLETIN,2011,56(18):1877-1883.
APA Xue WangXin,Zhang DeQing,Zhang GuanXin,&Zhu DaoBen.(2011).Colorimetric detection of glucose and an assay for acetylcholinesterase with amine-terminated polydiacetylene vesicles.CHINESE SCIENCE BULLETIN,56(18),1877-1883.
MLA Xue WangXin,et al."Colorimetric detection of glucose and an assay for acetylcholinesterase with amine-terminated polydiacetylene vesicles".CHINESE SCIENCE BULLETIN 56.18(2011):1877-1883.
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