ICCAS OpenIR
Highly sensitive detection of the hepatotoxin microcystin-LR by surface modification and bio-nanotechnology
Han, Jianhua; Zhang, Jianping; Xia, Yuetong; Jiang, Long
2011-11-05
Source PublicationCOLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
ISSN0927-7757
Volume391Issue:1-3Pages:184-189
AbstractA highly sensitive, portable method of detecting the hepatotoxin microcystin-LR (MC-LR) has been developed by a surface modification and gold nanoparticle (AuNP) technology. By using this technology a detection sensitivity of 0.1 pg mu L-1 MC-LR can be reached which meets the standard of World Health Organization (WHO) requirements for the drinking water (1 mu g mu L-1 MC-LR) and compatible with those of conventional techniques, such as high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA). Our method includes an inexpensive sensor, composed of a quartz crystal microbalance (QCM) along with gold nanoparticles (AuNPs), and a sandwiched immunoassay for rapid and in situ detection of MC-LR. The volume of analyte for once detection was generally less than 50 mu L The method specifically involves self-assembled monolayer formation on the QCM gold electrodes, immobilisation of MC-LR antibodies and AuNP signal amplification. It has been found that 30 nm is the optimal size for AuNPs which could effectively amplify the signals of MC. All the process was easily and promptly operated. Thus, a fast and convenient sandwiched immunoassay sensor has been established. (C) 2011 Elsevier B.V. All rights reserved.
KeywordQuartz Crystal Microbalance Microcystin-lr Immunoassay Gold Nanoparticles
DOI10.1016/j.colsurfa.2011.01.043
Indexed BySCI ; ISTP
Language英语
WOS IDWOS:000299068100026
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71470
Collection中国科学院化学研究所
Corresponding AuthorJiang, Long
AffiliationChinese Acad Sci, Colloids & Interfaces Lab, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Han, Jianhua,Zhang, Jianping,Xia, Yuetong,et al. Highly sensitive detection of the hepatotoxin microcystin-LR by surface modification and bio-nanotechnology[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2011,391(1-3):184-189.
APA Han, Jianhua,Zhang, Jianping,Xia, Yuetong,&Jiang, Long.(2011).Highly sensitive detection of the hepatotoxin microcystin-LR by surface modification and bio-nanotechnology.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,391(1-3),184-189.
MLA Han, Jianhua,et al."Highly sensitive detection of the hepatotoxin microcystin-LR by surface modification and bio-nanotechnology".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 391.1-3(2011):184-189.
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