ICCAS OpenIR
Visual optical discrimination and detection of microbial pathogens based on diverse interactions of conjugated polyelectrolytes with cells
Zhu, Chunlei; Yang, Qiong; Liu, Libing; Wang, Shu
2011
Source PublicationJOURNAL OF MATERIALS CHEMISTRY
ISSN0959-9428
Volume21Issue:22Pages:7905-7912
AbstractPathogen infections caused by Candida albicans (C. albicans) and Escherichia coli (E. coli) have led to extremely serious consequences. Cultures of blood samples, which are regarded as the diagnosis standard, often exhibit variable sensitivity and always take several days to detect certain pathogens. Here we demonstrate for the first time that a blend of two cationic conjugated polymers, between which fluorescence resonance energy transfer (FRET) can be induced, is used to detect and discriminate C. albicans and E. coli in a rapid, simple and visual way without the need for any complicated instrumentation. Coupled with general polymerase chain reaction (PCR) technology, the method can detect pathogens specifically independent of electrophoresis analyses and real-time PCR, which avoids utilizing detrimental nucleic dyes and expensive fluorophore-labeled primers. Our new cationic conjugated polymer blend has two unique features. Firstly, the assay is simple and rapid. Upon mixing fungi or bacteria with the polymer blend, one can trigger the signal response directly in solution using a fluorimeter, which may have a far-reaching impact on clinical diagnosis. Secondly, the most important characteristic of the assay is direct visualization under UV light, which makes it more convenient than other methods that require sophisticated instruments. The cationic conjugated polymer blend exhibits great potential as an ideal diagnostic agent for the identification of pathogens.
DOI10.1039/c0jm04424e
Indexed BySCI
Language英语
WOS IDWOS:000290912900011
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71464
Collection中国科学院化学研究所
Corresponding AuthorYang, Qiong
AffiliationChinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Chunlei,Yang, Qiong,Liu, Libing,et al. Visual optical discrimination and detection of microbial pathogens based on diverse interactions of conjugated polyelectrolytes with cells[J]. JOURNAL OF MATERIALS CHEMISTRY,2011,21(22):7905-7912.
APA Zhu, Chunlei,Yang, Qiong,Liu, Libing,&Wang, Shu.(2011).Visual optical discrimination and detection of microbial pathogens based on diverse interactions of conjugated polyelectrolytes with cells.JOURNAL OF MATERIALS CHEMISTRY,21(22),7905-7912.
MLA Zhu, Chunlei,et al."Visual optical discrimination and detection of microbial pathogens based on diverse interactions of conjugated polyelectrolytes with cells".JOURNAL OF MATERIALS CHEMISTRY 21.22(2011):7905-7912.
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