ICCAS OpenIR
Engineering a zinc binding site into the de novo designed protein DS119 with a beta alpha beta structure
Zhu, Cheng1; Zhang, Changsheng1; Liang, Huanhuan1; Lai, Luhua1,2
2011-12-01
Source PublicationPROTEIN & CELL
ISSN1674-800X
Volume2Issue:12Pages:1006-1013
AbstractFunctional proteins designed de novo have potential application in chemical engineering, agriculture and healthcare. Metal binding sites are commonly used to incorporate functions. Based on a de novo designed protein DS119 with a beta alpha beta structure, we have computationally engineered zinc binding sites into it using a home-made searching program. Seven out of the eight designed sequences tested were shown to bind Zn2+ with micromolar affinity, and one of them bound Zn2+ with 1:1 stoichiometry. This is the first time that metalloproteins with an alpha, beta mixed structure have been designed from scratch.
KeywordBeta Alpha Beta De Novo Design Folding Zinc-binding
DOI10.1007/s13238-011-1121-3
Indexed BySCI
Language英语
WOS IDWOS:000310522900009
PublisherHIGHER EDUCATION PRESS
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71400
Collection中国科学院化学研究所
Corresponding AuthorLai, Luhua
Affiliation1.Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
2.Peking Univ, Ctr Theoret Biol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Cheng,Zhang, Changsheng,Liang, Huanhuan,et al. Engineering a zinc binding site into the de novo designed protein DS119 with a beta alpha beta structure[J]. PROTEIN & CELL,2011,2(12):1006-1013.
APA Zhu, Cheng,Zhang, Changsheng,Liang, Huanhuan,&Lai, Luhua.(2011).Engineering a zinc binding site into the de novo designed protein DS119 with a beta alpha beta structure.PROTEIN & CELL,2(12),1006-1013.
MLA Zhu, Cheng,et al."Engineering a zinc binding site into the de novo designed protein DS119 with a beta alpha beta structure".PROTEIN & CELL 2.12(2011):1006-1013.
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