ICCAS OpenIR
Fluorescence study of interaction between an anionic conjugated polyelectrolyte and bovine serum albumin
Wang, Yani1; Dong, Jie1; Liu, Chuanzhen2; Bao, Biqing3; Wang, Lianhui3; Zhan, Xiaowei4; Yang, Huai1; Wang, Guojie1
2011-12-01
Source PublicationPOLYMER BULLETIN
ISSN0170-0839
Volume67Issue:9Pages:1907-1915
AbstractThe interaction between an anionic conjugated polyelectrolyte, poly[9,9-bis(3'-butyrate)fluoren-2,7-yl] sodium (BBS-PF), and bovine serum albumin was investigated by fluorescence spectroscopy. The emission of BBS-PF was effectively quenched by BSA with a quenching constant K (SV) of 3.1 x 10(7) L/mol when BSA was at nanomolar concentrations, but the emission increased when the concentration of BSA was at micromolar level. The excitation band of BBS-PF blue-shifted when the emission was quenched where the negatively charged BSA induced the aggregation of BBS-PF, yet the excitation band of BBS-PF red-shifted when the emission increased where the BSA acted as a surfactant and formed hydrophobic interaction with BBS-PF. BBS-PF could also quench BSA through energy transfer by resonance with a quenching constant K (SV) of 1.1 x 10(6) L/mol. The emission band changes of BSA reflected the conformation transitions of BSA from class II to class I and the binding of BBS-PF with BSA made the BSA more folded.
KeywordFluorescence Biosensor Conjugated Polyelectrolyte Protein Probe
DOI10.1007/s00289-011-0577-x
Indexed BySCI
Language英语
WOS IDWOS:000296732500015
PublisherSPRINGER
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71374
Collection中国科学院化学研究所
Corresponding AuthorWang, Guojie
Affiliation1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
2.Beijing BOE Display Technol Co Ltd, Beijing 100176, Peoples R China
3.Fudan Univ, Adv Mat Lab, Shanghai 200433, Peoples R China
4.Chinese Acad Sci, Inst Chem, CAS Key Lab Organ Solids, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yani,Dong, Jie,Liu, Chuanzhen,et al. Fluorescence study of interaction between an anionic conjugated polyelectrolyte and bovine serum albumin[J]. POLYMER BULLETIN,2011,67(9):1907-1915.
APA Wang, Yani.,Dong, Jie.,Liu, Chuanzhen.,Bao, Biqing.,Wang, Lianhui.,...&Wang, Guojie.(2011).Fluorescence study of interaction between an anionic conjugated polyelectrolyte and bovine serum albumin.POLYMER BULLETIN,67(9),1907-1915.
MLA Wang, Yani,et al."Fluorescence study of interaction between an anionic conjugated polyelectrolyte and bovine serum albumin".POLYMER BULLETIN 67.9(2011):1907-1915.
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